重组Anti-CD3 zeta抗体[BL-336-1B2] (ab243874)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-336-1B2] to CD3 zeta
- Suitable for: WB, IHC-P, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD3 zeta抗体[BL-336-1B2]
参阅全部 CD3 zeta 一抗 -
描述
兔单克隆抗体[BL-336-1B2] to CD3 zeta -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human CD3 zeta aa 150-164 (C terminal). The exact sequence is proprietary. NP_932170.1 and Gene ID 919.
Database link: P20963 -
阳性对照
- WB: K562, Jurkat, HeLa, SR, HEK293T, HepG2 and MOLT4 whole cell lysate. IHC-P: Human tonsil tissue, human lung cancer tissue. IP: Jurkat whole cell lysate.
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常规说明
This product is sold under License from Bethyl Laboratories, Inc.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading...
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纯化说明
Recombinant antibody was purified from cell culture supernatant. -
克隆
单克隆 -
克隆编号
BL-336-1B2 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab243874于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at an assay dependent concentration.
Use 20µl/mg lysate. |
说明 |
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WB
1/1000. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. Use 20µl/mg lysate. |
靶标
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功能
Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. -
疾病相关
Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens. -
序列相似性
Belongs to the CD3Z/FCER1G family.
Contains 3 ITAM domains. -
结构域
The ITAM domains mediate interaction with SHB. -
翻译后修饰
Phosphorylated on Tyr residues after T-cell receptor triggering. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 919 Human
- Omim: 186780 Human
- SwissProt: P20963 Human
- Unigene: 156445 Human
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别名
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
图片
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All lanes : Anti-CD3 zeta antibody [BL-336-1B2] (ab243874) at 1/1000 dilution
Lane 1 : Wild-type Jurkat cell lysate
Lane 2 : CD247 knockout Jurkat cell lysate
Lane 3 : MOLT-4 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 16 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CD3 zeta antibody [BL-336-1B2] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab243874 was shown to bind specifically to CD3 zeta. A band was observed at 16 kDa in wild-type Jurkat cell lysates with no signal observed at this size in CD247 knockout cell line ab273856 (knockout cell lysate ab273810). To generate this image, wild-type and CD247 knockout Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-CD3 zeta antibody [BL-336-1B2] (ab243874) at 1/1000 dilution
Lane 1 : K562 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : SR whole cell lysate
Lane 5 : HEK293T whole cell lysate
Lane 6 : HepG2 whole cell lysate
Lane 7 : MOLT4 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD247/CD3Z with ab243874. HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody, with DAB as substrate.
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CD247/CD3Z was immunoprecipitated from 1mg of Jurkat whole cell lysate with ab243874 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitate using ab264158 at a 1/1000 dilution.
Lane 1: ab243874 IP in Jurkat whole cell lysate.
Lane 2: Control IgG.Detection: Chemiluminescence.
Lysate prepared using NETN lysis buffer.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue, labeling CD247/CD3Z with ab243874. HRP-conjugated goat anti-rabbit IgG used as the secondary antibody. DAPI counterstain.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung cancer tissue labeling CD247/CD3Z with ab243874. HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody, with DAB as the substrate.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab243874 尚未被引用在任何文献中。