Name: Anti-CD3 epsilon antibody [EP449E]
Brand: RabMAb
SKU: ab52959
Rating: 5 (3 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP449E] to CD3 epsilon
Suitable for: WB, IHC-P, IP
Reacts with: Human

Carrier Free

产品名称

Anti-CD3 epsilon抗体[EP449E]
参阅全部 CD3 epsilon 一抗
描述

兔单克隆抗体[EP449E] to CD3 epsilon
宿主

Rabbit
特异性

ab52959 reacts with CD3 epsilon. It does not cross-react with other subunits of CD3.
经测试应用

适用于: WB, IHC-P, IPmore details
种属反应性

与反应: Human
免疫原

Synthetic peptide corresponding to Human CD3 epsilon aa 150-250. Derived from the cytoplasmic domain.
Database link: P07766
阳性对照
- Jurkat cell lysate and human spleen tissue. IP: Jurkat whole cell lysate.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

形式

Liquid
存放说明

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EP449E
同种型

IgG
研究领域

Alternative Versions
- Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)
- Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Jurkat membrane extract lysate (ab30130)
Recombinant Protein
- Recombinant human CD3 epsilon protein (Active) (ab167755)

The Abpromise guarantee

Abpromise™承诺保证使用ab52959于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB	(1)	1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P	(2)	1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP		1/20.

说明
WB 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/20.

功能

The CD3 complex mediates signal transduction.
序列相似性

Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain.
细胞定位

Membrane.
Target information above from: UniProt accession P07766 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 916 Human
- Omim: 186830 Human
- SwissProt: P07766 Human
- Unigene: 3003 Human
别名
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
- CD3e antigen epsilon polypeptide (TiT3 complex) antibody
- CD3E antigen epsilon polypeptide antibody
- CD3E antigen, epsilon subunit antibody
- CD3e molecule epsilon antibody
- CD3e molecule, epsilon (CD3 TCR complex) antibody
- CD3e molecule, epsilon (CD3-TCR complex) antibody
- CD3E_HUMAN antibody
- IMD18 antibody
- T cell antigen receptor complex epsilon subunit of T3 antibody
- T cell surface antigen T3/Leu 4 epsilon chain antibody
- T cell surface glycoprotein CD3 epsilon chain antibody
- T-cell surface antigen T3/Leu-4 epsilon chain antibody
- T-cell surface glycoprotein CD3 epsilon chain antibody
- T3E antibody
- TCRE antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

ab52959 at 1/250-1/500 dilution staining CD3 epsilon in human spleen by Immunohistochemistry, Paraffin embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-CD3 epsilon antibody [EP449E] (ab52959)

All lanes : Anti-CD3 epsilon antibody [EP449E] (ab52959) at 1/1000 dilution (purified)

Lane 1 : Jurkat( Human T cell leukemia T lymphocyte )membrane fraction cell lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) without membrane fraction cell lysate
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 23 kDa
Observed band size: 23 kDa

Exposure time: 20 seconds

Blocking/Dilution buffer and concentration: 5% NFDM/TBST
Immunoprecipitation - Anti-CD3 epsilon antibody [EP449E] (ab52959)

Purified ab52959 at 1/20 dilution (1µg) immunoprecipitating CD3 epsilon in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab52959 + Jurkat whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52929 in Jurkat whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 23 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

ab52959 showing positive staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

ab52959 showing positive staining in Normal spleen tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

ab52959 showing negative staining in Normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

ab52959 showing negative staining in Normal kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

ab52959 showing negative staining in Skeletal muscle tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-CD3 epsilon antibody [EP449E] (ab52959)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

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发表研究结果有使用 ab52959？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab52959 被引用在 15 文献中.

Simonson PD et al. Tyramide-conjugated DNA barcodes enable signal amplification for multiparametric CODEX imaging. Commun Biol 5:627 (2022). PubMed: 35754060
Schaadt NS et al. Graph-based description of tertiary lymphoid organs at single-cell level. PLoS Comput Biol 16:e1007385 (2020). PubMed: 32084130
Kim D et al. Somatic mTOR mutation in clonally expanded T lymphocytes associated with chronic graft versus host disease. Nat Commun 11:2246 (2020). PubMed: 32382059
Rao A et al. Cytokines regulate the antigen-presenting characteristics of human circulating and tissue-resident intestinal ILCs. Nat Commun 11:2049 (2020). PubMed: 32341343
Yang X et al. Misdiagnosis of primary pleural DLBCL as tuberculosis: A case report and literature review. Mol Clin Oncol 8:729-732 (2018). PubMed: 29732155

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1-4 of 4 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Skin)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate

Permeabilization

Specification

Skin

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 17 2022

Application

Western blot

Sample

Human Cell lysate - whole cell (T-cells)

Gel Running Conditions

Reduced Denaturing (4-20% Tris HCl)

Loading amount

7.69 µg

Specification

T-cells

Blocking step

Licor intercept TBS as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 13 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (distal colon)

Specification

distal colon

Fixative

Paraformaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate buffer

Permeabilization

Blocking step

Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Isabel Romero Calvo

Verified customer

提交于 Jan 11 2013

Question

Antikörper, der humane T Zellen erkennt aber NICHT T Zellen aus der Ratte in IHC-P.

Abcam community

Verified customer

Asked on Sep 22 2011

Answer

Vielen dank fuer Ihren Anruf. Ich habe nun ein paar Antikoerper fuer Sie herrausgesucht, die nur humane T Zellen erkennen und nicht T Zellen aus der Ratte und fuer IHC-P getestet und garantiert sind. Bitte folgen Sie den jeweiligen Links um zu den Datenblaetern mit mehr Informatinen zugelangen. ab32327 Vielen Dank für Ihren Anruf. Ich habe nun ein paar Antikörper für Sie herausgesucht, die nur humane T Zellen erkennen und nicht T Zellen aus der Ratte und für IHC-P getestet und garantiert sind. Bitte folgen Sie den jeweiligen Links um zu den Datenblättern mit mehr Informationen zugelangen. ab32327 Click here (or use the following: https://www.abcam.com/CD3-epsilon-antibody-E292-ab32327.html). ab33524 Click here (or use the following: https://www.abcam.com/CD3-epsilon-antibody-PS1-ab33524.html). ab52959 Click here (or use the following: https://www.abcam.com/CD3-epsilon-antibody-EP449E-ab52959.html). ab109531 Click here (or use the following: https://www.abcam.com/CD3D-antibody-EP4426-ab109531.html). Ich hoffe, diese Liste hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder zu melden, falls Sie weitere Fragen haben. Click here (or use the following: https://www.abcam.com/CD3-epsilon-antibody-E292-ab32327.html). ab33524 Click here (or use the following: https://www.abcam.com/CD3-epsilon-antibody-PS1-ab33524.html). ab52959 Click here (or use the following: https://www.abcam.com/CD3-epsilon-antibody-EP449E-ab52959.html). ab109531 Click here (or use the following: https://www.abcam.com/CD3D-antibody-EP4426-ab109531.html). Ich hoffe, diese Liste hilft Ihnen weiter. Bitte zoegern Sie nicht, sich wioeder zu melden, falls Sie weitere Fragen haben.

Abcam Scientific Support

回复于 Sep 22 2011

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

特异性

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (15)

客户评价及客户问答

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 epsilon antibody [EP449E]

Western blot abreview for Anti-CD3 epsilon antibody [EP449E]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 epsilon antibody [EP449E]