Anti-CD3抗体[RM0027-3B19] (ab56313)

Merci pour cesprécisions.

Nous sommes désolés que le produit ab56313 que vous avez reçu ne fonctionne pas comme attendu. Ce produit est actuellement étudié,retesté et recaractérisédansle butdemaintenir un hautniveau contrôle qualité.
Comme convenu j'ai mis en place l'envoi d'une unité gratuite d'anti-CD3 ab5690 en remplacement du ab56313. Le numéro de commande est ****. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

N'hésitez pas à nous contacter lors d'une prochaine occasion.

Merci de nous avoir contactés et merci beaucoup pour les détails de votre protocole. Ces informations sont très importantes pour nous car elles nous permettent de rechercher la source du problème rencontré avec cet anticorps et de prendre les dispositions nécessaires pour assurer une bonne qualité de nos produits.

Nous sommes désolés que le produit ab56313 que vous avez reçu ne fonctionne pas comme attendu.

Le seul lot actuellement en stock est celui que vous avez reçu (GR53072). Préférez-vous recevoir unautre anti-CD3tel que :
- le polyclonal de lapin ab5690 : https://www.abcam.com/ab5690
-lemonoclonal de lapin ab16669 : https://www.abcam.com/ab16669
-le polyclonal de lapin ab16044 : https://www.abcam.com/ab16044
-ou un autreanti-CD3.Le lien vers la liste complète des 7 anti-CD3 testé en IHC-P et chez la souris est : https://www.abcam.com/index.html?pageconfig=searchresults&search=CD3&pt=1&sk=react&sv=2&sn=Mouse&l=3&fViewMore=1

Je peux égalementéditer un avoir ou un remboursement contre la commande originale.

Merci de m'indiquer la résolution qui vous conviendrait le mieux.

Thank you for your inquiry.


Please find the recommended IHC protocol below:


1. Dewax and Rehydration: Place the slides in a rack and sequentially pass the rack through following solutions: Histoclear or Xylene twice, 10 minutes each; and then a graded series of 100%, 90%, 70%, and 50% ethanol, 2 minutes each.


2. Wash the slides with tap water for 5 minutes and once with DI water.


3. Antigen Retrieval: treat the sections with 20ug/ml proteinase K in PBS for 15-25 minutes at room temperature.


4. Wash the slides with tap water for 5 minutes and quench the endogenous peroxidase with 3% H2O2 in DI water for 30 minutes


5. Wash the slides with tap water for 5mins and soaking in PBS-Tween20 (0.1%)


6. Block the slides with blocking buffer (25%bovine serum in PBS-Tween20) for at least 10minutes


7. Then incubate the specimen with primary antibody (ab56313, dissolved with 200ul PBS) at 1:25-100 dilutions overnight at 4 degree (or room temperature if the signal is too weak).


8. Wash the slides with rotation in PBS-Tween20 for 3 times, 5 minutes each.


9. Incubate the specimen with rabbit-anti rat secondary antibody for 30minutes at room temperature.


10. Wash the slides with rotation in PBS-Tween 20 for 3 times, 5 minutes each.


11. Incubate the specimen with Polymer-HRP labeled anti rabbit for 30 minutes at room temperature.


12. Wash the slides with rotation in PBS-Tween20 for 3 times, 5 minutes each.


13. Perform DAB Color development.


14. Wash with tap water and count stain the nuclear with hematoxylin.


Note: 1) If you use buffered formalin fixatives, we recommend that fixation should be done no longer than overnight (4

degree preferred). 2) This protocol is for reference only and the final condition should be optimized by the end user.


I hope this information halps. Please contact us with any other questions.

Thank you for your reply.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.