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Immunology Adaptive Immunity T Cells CD

Anti-CD3抗体[PS1] (ab699)

  • Datasheet
  • SDS
Reviews (12)Q&A (12)References (25)

Product price, shipping and contact information

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Key features and details

  • Mouse monoclonal [PS1] to CD3
  • Suitable for: IHC-Fr, IHC-P, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG2a

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概述

  • 产品名称

    Anti-CD3抗体[PS1]
    参阅全部 CD3 一抗
  • 描述

    小鼠单克隆抗体[PS1] to CD3
  • 宿主

    Mouse
  • 特异性

    This antibody, when used in conjunction with UCHL-1, is regarded as a reliable pan T-cell marker for immunophenotyping of lymphomas (See reference). Exceptions include some of the more aggressive, high-grade T cell lymphomas, which may not express detectable antigen. This antibody does not react with B-cells.
  • 经测试应用

    适用于: IHC-Fr, IHC-P, ICC/IF, WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Fusion protein corresponding to CD3. Prokaryotic fusion protein corresponding to the epsilon chain of CD3

  • 阳性对照

    • Tonsil or T-cell lymphoma. IHC-P: Human colon tissue and colorectal mucosa tissue. IHC-Fr: Human skin tissue.
  • 常规说明

    This antibody works well when used in combination with ab972 - Antigen retrieval solution - heat mediated high pH 9.5.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: Proprietary preservative
    Constituents: PBS, Carrier protein

    Proprietary preservative that is not sodium azide or thimerosal, protein carrier.
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 克隆

    单克隆
  • 克隆编号

    PS1
  • 骨髓瘤

    unknown
  • 同种型

    IgG2a
  • 轻链类型

    unknown
  • 研究领域

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Mouse CD3 protein (Fc Chimera) (ab220543)
    • Recombinant Human CD3 epsilon protein (Fc Chimera His Tag) (ab220590)
  • Related Products

    • Human CD3 peptide (ab16206)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab699于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr (1)
Use at an assay dependent concentration.
IHC-P (3)
Use at an assay dependent concentration.
ICC/IF (3)
Use at an assay dependent concentration.
WB (4)
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa. PubMed: 21654633
说明
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa. PubMed: 21654633

靶标

  • 功能

    The CD3 complex mediates signal transduction.
  • 疾病相关

    Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • 序列相似性

    Contains 1 ITAM domain.
  • 细胞定位

    Membrane.
  • Target information above from: UniProt accession P04234 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 915 Human
    • Entrez Gene: 916 Human
    • Entrez Gene: 917 Human
    • Entrez Gene: 919 Human
    • Omim: 186740 Human
    • Omim: 186780 Human
    • Omim: 186790 Human
    • Omim: 186830 Human
    • SwissProt: P04234 Human
    • SwissProt: P07766 Human
    • SwissProt: P09693 Human
    • SwissProt: P20963 Human
    • Unigene: 504048 Human
    see all
  • 别名

    • 4930549J05Rik antibody
    • A430104F18Rik antibody
    • AW552088 antibody
    • Cd247 antibody
    • CD247 antigen antibody
    • CD247 antigen, zeta subunit antibody
    • CD247 molecule antibody
    • CD3 antibody
    • CD3 antigen, delta subunit antibody
    • CD3 delta antibody
    • CD3 epsilon antibody
    • CD3 eta antibody
    • CD3 gamma antibody
    • CD3 molecule delta polypeptide antibody
    • CD3 molecule, epsilon polypeptide antibody
    • CD3 molecule, gamma polypeptide antibody
    • CD3 zeta antibody
    • CD3-DELTA antibody
    • CD3d antibody
    • CD3D antigen delta polypeptide antibody
    • CD3d antigen, delta polypeptide (TiT3 complex) antibody
    • CD3d molecule delta antibody
    • CD3d molecule delta CD3 TCR complex antibody
    • CD3d molecule, delta (CD3-TCR complex) antibody
    • CD3D_HUMAN antibody
    • CD3E antibody
    • CD3e antigen antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3e antigen, epsilon polypeptide (TiT3 complex) antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule epsilon CD3 TCR complex antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3epsilon antibody
    • CD3G antibody
    • CD3g antigen antibody
    • CD3G antigen gamma polypeptide antibody
    • CD3g antigen, gamma polypeptide (TiT3 complex) antibody
    • CD3g molecule gamma antibody
    • CD3g molecule gamma CD3 TCR complex antibody
    • CD3g molecule, gamma (CD3-TCR complex) antibody
    • CD3H antibody
    • CD3Q antibody
    • CD3Z antibody
    • CD3zeta antibody
    • Ctg3 antibody
    • FLJ17620 antibody
    • FLJ17664 antibody
    • FLJ18683 antibody
    • FLJ79544 antibody
    • FLJ94613 antibody
    • IMD19 antibody
    • Leu-4 antibody
    • MGC138597 antibody
    • OKT3, delta chain antibody
    • OTTHUMP00000032544 antibody
    • T cell receptor antibody
    • T cell receptor T3 delta chain antibody
    • T cell receptor T3 gamma chain antibody
    • T cell receptor T3 zeta chain antibody
    • T cell receptor zeta chain antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 antibody
    • T cell surface glycoprotein CD3 delta chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T cell surface glycoprotein CD3 gamma chain antibody
    • T cell surface glycoprotein CD3 zeta chain antibody
    • T-cell antigen receptor complex, delta subunit of T3 antibody
    • T-cell antigen receptor complex, epsilon subunit of T3 antibody
    • T-cell antigen receptor complex, gamma subunit of T3 antibody
    • T-cell antigen receptor complex, zeta subunit of CD3 antibody
    • T-cell receptor T3 delta chain antibody
    • T-cell receptor T3 gamma chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 delta chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface glycoprotein CD3 gamma chain antibody
    • T3 antibody
    • T3d antibody
    • T3e antibody
    • T3g antibody
    • T3z antibody
    • TCRE antibody
    • TCRk antibody
    • Tcrz antibody
    • TCRzeta antibody
    see all

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (25)

发表研究结果有使用 ab699?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab699 被引用在 25 文献中.

  • Zhan X  et al. Glioma stem-like cells evade interferon suppression through MBD3/NuRD complex-mediated STAT1 downregulation. J Exp Med 217:N/A (2020). PubMed: 32181805
  • Liu Z  et al. IL-21 promotes osteoblastic differentiation of human valvular interstitial cells through the JAK3/STAT3 pathway. Int J Med Sci 17:3065-3072 (2020). PubMed: 33173427
  • Wang B  et al. Loss of survival advantage for deficient mismatch repair in patients with advanced colorectal cancer may be caused by changes in prognostic value of CD8+T cell. World J Surg Oncol 18:196 (2020). PubMed: 32767974
  • Wang Y  et al. Expression and clinical significance of PD-L1, B7-H3, B7-H4 and VISTA in craniopharyngioma. J Immunother Cancer 8:N/A (2020). PubMed: 32958683
  • Liang H  et al. The proatherosclerotic function of indoleamine 2, 3-dioxygenase 1 in the developmental stage of atherosclerosis. Signal Transduct Target Ther 4:23 (2019). PubMed: 31637003
View all Publications for this product

客户评价及客户问答

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1-10 of 24 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [PS1]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Sarcoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
Human Sarcoma
Blocking step
innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 09 2015

Western blot abreview for Anti-CD3 antibody [PS1]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gels)
Sample
Human Cell lysate - whole cell (Jurkats and PBMCS)
Specification
Jurkats and PBMCS
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 19 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-CD3 antibody [PS1]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Lymphocytes)
Specification
Lymphocytes
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 06 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [PS1]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 06 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-CD3 antibody [PS1]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human peripheral blood mononuclear cells)
Specification
Human peripheral blood mononuclear cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 25 2013

Western blot abreview for Anti-CD3 antibody [PS1]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Spleen whole extract)
Loading amount
100 µg
Specification
Spleen whole extract
Gel Running Conditions
Reduced Denaturing (Tris-Glycine -10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 28 2012

Western blot abreview for Anti-CD3 antibody [PS1]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Peripheral blood mononuclear cells)
Loading amount
20 µg
Specification
Peripheral blood mononuclear cells
Gel Running Conditions
Reduced Denaturing (Tris-Glycine -10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 31 2012

Western blot abreview for Anti-CD3 antibody [PS1]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human peripheral blood mononuclear cells)
Loading amount
25 µg
Specification
Human peripheral blood mononuclear cells
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 30 2012

Question

Interested in CD3 ab without sodium azide.

Read More

Abcam community

Verified customer

Asked on May 07 2013

Answer

Here is a link to our protocol for sodium azide removal

https://www.abcam.com/index.html?pageconfig=resource&rid=13869

Read More

Abcam Scientific Support

回复于 May 07 2013

Question

I ordered the following antibody which I will be using for IHC-P:

Anti-CD3 antibody [PS1] (ab699)

To use the corresponding isotype control with the correct concentration I need to know how the Anti-CD3 antibody is concentrated.

Could you please e-mail me the concentration or if the exact concentration is not known which concentration it roughly has?

Thank you in advance.

Read More

Abcam community

Verified customer

Asked on Jan 08 2013

Answer

Vielen Dank für Ihre Anfrage.

Die Konzentration für den ab699 Anti-CD3 antibody [PS1] mit dem Lot GR95849-1, welches Sie erhalten haben, beträgt 80ug/ml.

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

回复于 Jan 08 2013

1-10 of 24 Abreviews or Q&A

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  • 染色质及细胞核信号转导研究
  • 免疫学
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