AB86883

Anti-CD3 抗体 [OKT3]

Anti-CD3 antibody [OKT3]

Lab Essentials
了解详情

5

(1 Review)

|

(14 Publications)

Mouse Monoclonal CD3d antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Cell preparation containing T Cell protein.

查看别名

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

不同偶联物与剂型 (8)

660 APC

APC Anti-CD3 epsilon antibody [OKT3]
660 APC

APC Anti-CD3 epsilon antibody [OKT3]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD3 antibody [OKT3]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD3 antibody [OKT3]
Biotin

Biotin Anti-CD3 antibody [OKT3]
519 FITC

FITC Anti-CD3 epsilon antibody [OKT3]
578 PE

PE Anti-CD3 epsilon antibody [OKT3]
704 redFluor™ 710

redFluor™ 710 Anti-CD3 antibody [OKT3]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

OKT3

亚型

IgG2a

轻链类型

kappa

不含载体蛋白

No

反应种属

Human

应用

Flow Cyt

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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替代产品

Primary Antibodies

AB5690

Anti-CD3 epsilon antibody

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody (AB5690)

4
46

Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

4
80

Primary Antibodies

AB21703

Anti-CD3 epsilon antibody [SP7], prediluted

BOND RX™ Validated|RabMAb|Lab Essentials|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7], prediluted (AB21703)

5
1

Primary Antibodies

AB246799

Anti-CD3 antibody [SP162] - Low endotoxin, Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (AB246799)

0
0

Primary Antibodies

AB245731

Anti-CD3 antibody [SP162] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] - BSA and Azide free (AB245731)

5
3

Primary Antibodies

AB135372

Anti-CD3 antibody [SP162]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (AB135372)

4
10

Primary Antibodies

AB16044

Anti-CD3 antibody

Immunoprecipitation - Anti-CD3 antibody (AB16044)

4
2

搭配使用产品

Primary Antibodies

AB184787

Anti-Caspase-3 antibody [EPR18297]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [EPR18297] (AB184787)

3
3

Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

4
80

Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

0
0

Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

0
0

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FuncSAct" : {"fullname" : "Functional Studies (Act)", "shortname":"FuncS (Act)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "FuncSAct-species-checked": "notRecommended", "FuncSAct-species-dilution-info": "", "FuncSAct-species-notes": "<p>(Activation)</p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate

运输条件

Blue Ice

推荐的短期储存时间

Up to 12 months

推荐的短期储存条件

+4°C

推荐的长期储存条件

-20°C

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).

See full target information CD3D

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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1597779 PubMed40458393

2025

The novel role of Yin Yang 1 in acute rejection of liver allografts through activation of dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Chen,Jie Wang,Liping Hong,Hongtao Wang,Wubing He,Lihong Chen

Journal for immunotherapy of cancer 12: PubMed38688579

2024

ScRNA-seq reveals novel immune-suppressive T cells and investigates CMV-TCR-T cells cytotoxicity against GBM.

Applications

Unspecified application

Species

Unspecified reactive species

Xinmiao Long,Zuping Zhang,Yuzhe Li,Kun Deng,Wei Gao,Meng Huang,Xiangyu Wang,Xiang Lin,Xiaoling She,Yiming Zhao,Minfu Zhang,Cheng Huang,Shiyi Wang,Yinfei Du,Peng Du,Shuai Chen,Qing Liu,Minghua Wu

Methods in molecular biology (Clifton, N.J.) 2789:109-120 PubMed38506996

2024

Detection of Intracellular Complement Activation by Nanoparticles in Human T Lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Ilinskaya,Ankit Shah,Andrew Van Dusen,Marina A Dobrovolskaia

Nature communications 14:2071 PubMed37045832

2023

Low-dose IL-2 enhances the generation of IL-10-producing immunoregulatory B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Akimichi Inaba,Zewen Kelvin Tuong,Tian X Zhao,Andrew P Stewart,Rebeccah Mathews,Lucy Truman,Rouchelle Sriranjan,Jane Kennet,Kourosh Saeb-Parsy,Linda Wicker,Frank Waldron-Lynch,Joseph Cheriyan,John A Todd,Ziad Mallat,Menna R Clatworthy

Biomedical reports 16:3 PubMed34820126

2021

Evaluation of skin sensitization based on interleukin-2 promoter activation in Jurkat cells.

Applications

Unspecified application

Species

Unspecified reactive species

Taichi Nagahata,Yoshio Tsujino,Eiji Takayama,Haruka Hikasa,Ayano Satoh

Frontiers in immunology 12:679398 PubMed34177922

2021

High Mobility Group Box 1 Contributes to the Acute Rejection of Liver Allografts by Activating Dendritic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Chen,Wenmin Zhang,Hui Bao,Wubing He,Lihong Chen

Nature methods 18:84-91 PubMed33398190

2021

Cell surface thermal proteome profiling tracks perturbations and drug targets on the plasma membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Mathias Kalxdorf,Ina Günthner,Isabelle Becher,Nils Kurzawa,Sascha Knecht,Mikhail M Savitski,H Christian Eberl,Marcus Bantscheff

Cellular signalling 58:20-33 PubMed30831195

2019

Regulation of endothelial cell survival and death by the MAP kinase/ERK kinase kinase 3 - glyceraldehyde-3-phosphate dehydrogenase signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Q Li,Andy Ngo,Peter Hoffmann,Antonio Ferrante,Charles S Hii

Nanomedicine : nanotechnology, biology, and medici 17:266-275 PubMed30794962

2019

Nanoparticle physicochemical properties determine the activation of intracellular complement.

Applications

Unspecified application

Species

Unspecified reactive species

Anna N Ilinskaya,Ankit Shah,Alan E Enciso,King C Chan,Jan A Kaczmarczyk,Josip Blonder,Eric E Simanek,Marina A Dobrovolskaia

The journals of gerontology. Series A, Biological sciences and medical sciences 71:1124-30 PubMed26525092

2015

Association Between Circulating Osteogenic Progenitor Cells and Disability and Frailty in Older Persons: The Nepean Osteoporosis and Frailty Study.

Applications

Unspecified application

Species

Unspecified reactive species

Piumali Gunawardene,Sandra Bermeo,Christopher Vidal,Ahmed Al-Saedi,Philip Chung,Derek Boersma,Steven Phu,Izabella Pokorski,Pushpa Suriyaarachchi,Oddom Demontiero,Gustavo Duque

View all publications

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