Anti-CD3抗体[MEM-57] (ab8090)
![Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)](https://www.abcam.cn/ps/products/8/ab8090/Images/ab8090-199441-anti-cd3-antibody-mem-57-flow-cytometry.jpg "Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)")
Key features and details
- Mouse monoclonal [MEM-57] to CD3
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-CD3抗体[MEM-57]
参阅全部 CD3 一抗 -
描述
小鼠单克隆抗体[MEM-57] to CD3 -
宿主
Mouse -
特异性
This antibody reacts with epsilon-gamma and epsilon-delta dimers of human CD3 complex, a part of a bigger multisubunit complex of the T cell receptor for antigen (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes. It reacts more strongly with blood gamma-delta T lymphocytes than with alpha-beta T lymphocytes. -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD3. Human thymocytes and T lymphocytes
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常规说明
Activates T cells in vitro. This antibody is a highly effective immunosuppressive agent. In this it is similar to OKT3.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
This product has been purified from tissue culture supernatant. -
Primary antibody说明
Activates T cells in vitro. This antibody is a highly effective immunosuppressive agent. In this it is similar to OKT3. -
克隆
单克隆 -
克隆编号
MEM-57 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
应用
图片
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Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)
Human peripheral blood lymphocytes stained with ab8090 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.
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![Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)](https://www.abcam.cn/ps/products/8/ab8090/Images/ab8090-455640-anti-cd3-antibody-mem-57-flow-cytometry-peripheral-blood-human.jpg)
Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)Flow cytometry analysis of human peripheral whole blood labeling CD3 with ab8090 at 0.33 μg/mL (GAM APC).
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![Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)](https://www.abcam.cn/ps/products/8/ab8090/Images/ab8090-455639-anti-cd3-antibody-mem-57-flow-cytometry-histogram-peripheral-blood-human.jpg)
Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)Flow cytometry analysis of human peripheral whole blood labeling CD3 with ab8090 at 0.33 μg/mL (GAM APC). Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed).
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![Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)](https://www.abcam.cn/ps/products/8/ab8090/Images/ab8090-279886-anti-cd3-antibody-mem-57-flow-cytometry.jpg)
Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD3 (MEM-57) Dy161. Gated on singlets.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab8090 被引用在 8 文献中.
- Garrett SM et al. Induction of a Th17 Phenotype in Human Skin-A Mimic of Dermal Inflammatory Diseases. Methods Protoc 2:N/A (2019). PubMed: 31164624
- Savitski MM et al. Multiplexed Proteome Dynamics Profiling Reveals Mechanisms Controlling Protein Homeostasis. Cell 173:260-274.e25 (2018). PubMed: 29551266
- Fehrholz M et al. Impact of the New Generation Reconstituted Surfactant CHF5633 on Human CD4+ Lymphocytes. PLoS One 11:e0153578 (2016). PubMed: 27077658
- Lipp AM et al. Lck mediates signal transmission from CD59 to the TCR/CD3 pathway in Jurkat T cells. PLoS One 9:e85934 (2014). Human . PubMed: 24454946
- Giese S & Marsh M Tetherin can restrict cell-free and cell-cell transmission of HIV from primary macrophages to T cells. PLoS Pathog 10:e1004189 (2014). Flow Cyt . PubMed: 24991932
- Bergamini G et al. A selective inhibitor reveals PI3K? dependence of T(H)17 cell differentiation. Nat Chem Biol 8:576-82 (2012). PubMed: 22544264
- Hilgert I et al. Therapeutic in vivo use of the A1-CD3 monoclonal antibody. Transplantation 55:435-8 (1993). PubMed: 8434399
- Horejsà V et al. Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha) 34:23-34 (1988). PubMed: 2968928