重组Anti-CD276抗体[SP265] - BSA and Azide free (ab272011)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP265] to CD276 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD276抗体[SP265] - BSA and Azide free
参阅全部 CD276 一抗 -
描述
兔单克隆抗体[SP265] to CD276 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, Flow Cyt, WBmore details
不适用于: IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Hs 700T cell lysate. Flow cytometry: PC-3 cells. ICC/IF: HEK293 and LNCaP cells
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常规说明
ab272011 is the carrier-free version of ab227679.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A/G purified -
克隆
单克隆 -
克隆编号
SP265 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-CD276 antibody [SP265] - C-terminal (ab227679)
- PE Anti-CD276 antibody [SP265] (C-terminal) (ab305811)
- APC Anti-CD276 antibody [SP265] (C-terminal) (ab305812)
- HRP Anti-CD276 antibody [SP265] (C-terminal) (ab305813)
- Alexa Fluor® 488 Anti-CD276 antibody [SP265] - C-terminal (ab309918)
- Alexa Fluor® 647 Anti-CD276 antibody [SP265] - C-terminal (ab310286)
- Alexa Fluor® 594 Anti-CD276 antibody [SP265] - C-terminal (ab310747)
- Alexa Fluor® 555 Anti-CD276 antibody [SP265] - C-terminal (ab312274)
- Alexa Fluor® 568 Anti-CD276 antibody [SP265] - C-terminal (ab312767)
- Alexa Fluor® 750 Anti-CD276 antibody [SP265] - C-terminal (ab321449)
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Compatible Secondaries
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Isotype control
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KO cell lines
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272011于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100.
|
|
Flow Cyt |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
|
说明 |
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ICC/IF
1/100. |
Flow Cyt
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa. |
靶标
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功能
May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. -
组织特异性
Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta. -
序列相似性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 80381 Human
- Entrez Gene: 102657 Mouse
- Entrez Gene: 315716 Rat
- Omim: 605715 Human
- SwissProt: Q5ZPR3 Human
- SwissProt: Q8VE98 Mouse
- SwissProt: Q7TPB4 Rat
- Unigene: 77873 Human
see all -
别名
- 4Ig B7 H3 antibody
- 4Ig-B7-H3 antibody
- AU016588 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
This data was developed using the same antibody clone in a different buffer formulation (ab227679).
ab227679 staining CD276 in wild-type HEK293 cells (top panel) and CD276 knockout HEK293 cells (ab266658) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab227679 at 1/100 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
Immunocytochemistry/ Immunofluorescence - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
Immunocytochemistry/ Immunofluorescence analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling CD276 with purified ab227679 at 1/25 (9.24 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227679). -
Flow cytometric analysis of PC-3 (human prostate adenocarcinoma cell line) cell line labeling CC276 with ab227679 at 1/400 (green) compared with a rabbit isotype control (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227679).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
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