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重组RabMAb

重组Anti-CD272/BTLA抗体[EPR29068-115] - BSA and Azide free (ab317828)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] - BSA and Azide free (ab317828)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] - BSA and Azide free (ab317828)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] - BSA and Azide free (ab317828)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] - BSA and Azide free (ab317828)
  • Multiplex immunohistochemistry - Anti-CD272/BTLA antibody [EPR29068-115] - BSA and Azide free (ab317828)
  • Anti-CD272/BTLA antibody [EPR29068-115] - BSA and Azide free (ab317828)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR29068-115] to CD272/BTLA - BSA and Azide free
  • Suitable for: IHC-P, mIHC
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-CD272/BTLA抗体[EPR29068-115] - BSA and Azide free
    参阅全部 CD272/BTLA 一抗

  • 描述

    兔单克隆抗体[EPR29068-115] to CD272/BTLA - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, mIHCmore details
    不适用于: Flow Cyt,ICC/IF,IP or WB

  • 种属反应性

    与反应: Mouse
    不与反应: Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Mouse spleen and Mouse lung. MIHC: Mouse spleen tissue.

  • 常规说明

    ab317828 is the carrier-free version of ab317827

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    pH: 7.20
    Constituent: 100% PBS

  • 无载体

    是
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR29068-115

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317828于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
mIHC
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
mIHC
Use at an assay dependent concentration.
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IP or WB.

靶标

  • 功能

    Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response.

  • 序列相似性

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 翻译后修饰

    Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.
    N-glycosylated.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • B and T lymphocyte associated protein antibody
      • B and T lymphocyte attenuator antibody
      • B and T lymphocyte associated antibody
      • B- and T-lymphocyte attenuator antibody
      • B- and T-lymphocyte-associated protein antibody
      • BTLA antibody
      • BTLA_HUMAN antibody
      • BTLA1 antibody
      • CD272 antibody
      • CD272 antigen antibody
      • FLJ16065 antibody
      • MGC129743 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] - BSA and Azide free (ab317828)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] - BSA and Azide free (ab317828)

      This data was developed using ab317827, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD272/BTLA with ab317827 at 1/100 (4.92 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining in mouse spleen.
      The section was incubated with ab317827 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] - BSA and Azide free (ab317828)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] - BSA and Azide free (ab317828)

      This data was developed using ab317827, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling CD272/BTLA with ab317827 at 1/100 (4.92 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining in immune cells of mouse lung.
      The section was incubated with ab317827 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] - BSA and Azide free (ab317828)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] - BSA and Azide free (ab317828)

      This data was developed using ab317827, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling CD272/BTLA with ab317827 at 1/100 (4.92 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Negative control: No staining in mouse cerebrum.
      The section was incubated with ab317827 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] - BSA and Azide free (ab317828)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] - BSA and Azide free (ab317828)

      This data was developed using ab317827, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling CD272/BTLA with ab317827 at 1/100 (4.92 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Negative control: No staining in mouse cardiac muscle.
      The section was incubated with ab317827 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

    • Multiplex immunohistochemistry - Anti-CD272/BTLA antibody [EPR29068-115] - BSA and Azide free (ab317828)
      Multiplex immunohistochemistry - Anti-CD272/BTLA antibody [EPR29068-115] - BSA and Azide free (ab317828)

      This data was developed using ab317827, the same antibody clone in a different buffer formulation.

      Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spleen tissue staining CD272/BTLA with ab317827 at a 1:1000 (0.44 ug/ml) dilution, ab317827 anti-BTLA used at 1:100 (4.92 ug/ml) dilution and ab16669 anti-CD3 used at a 1:150 (0.06 ug/ml) dilution.

      Panel A: merged staining of anti-CD19 (magenta; Opal™690), anti-BTLA (green; Opal™520) and anti-CD3 (yellow; Opal™570) on mouse spleen.
      Panel B: anti-CD19 staining B lymphocytes in mouse spleen.
      Panel C: anti-BTLA staining B lymphocytes and T lymphocytes in mouse spleen.
      Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
      Nuclear DNA was labeled with DAPI (shown in blue).

      The section was incubated in three rounds of staining: in the order of ab245235, ab317827 and ab16669 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

      Nuclear counter stain with DAPI.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-CD272/BTLA antibody [EPR29068-115] - BSA and Azide free (ab317828)
      Anti-CD272/BTLA antibody [EPR29068-115] - BSA and Azide free (ab317828)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab317828？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab317828 尚未被引用在任何文献中。

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