Anti-CD27抗体(ab175403)
Key features and details
- Rabbit polyclonal to CD27
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-CD27抗体
参阅全部 CD27 一抗 -
描述
兔多克隆抗体to CD27 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant full length protein within Human CD27 aa 20-191. The exact sequence is proprietary.
Database link: P26842 -
阳性对照
- WB: Raji, HeLa, Jurkat and HL-60 cell lysates. Mouse thymus tissue lysate. IHC: rat and mouse spleen tissues. IF: Jurkat cells, rat and mouse thymus tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.09% Sodium azide
Constituents: 49.91% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175403于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/50 - 1/200.
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| WB |
1/500 - 1/2000. Predicted molecular weight: 29 kDa.
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| IHC-P |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
ICC/IF
1/50 - 1/200. |
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WB
1/500 - 1/2000. Predicted molecular weight: 29 kDa. |
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IHC-P
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. -
组织特异性
Found in most T-lymphocytes. -
序列相似性
Contains 3 TNFR-Cys repeats. -
翻译后修饰
Phosphorylated and O-glycosylated. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 939 Human
- Entrez Gene: 21940 Mouse
- Entrez Gene: 500318 Rat
- Omim: 186711 Human
- SwissProt: P26842 Human
- SwissProt: P41272 Mouse
- Unigene: 355307 Human
- Unigene: 367714 Mouse
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别名
- CD 27 antibody
- CD27 antibody
- CD27 antigen antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody (ab175403)
Paraffin-embedded mouse spleen tissue stained for CD27 using ab175403 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-CD27 antibody (ab175403)Immunofluorescence staining of mouse thymus tissue stained for CD27 with ab175403 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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Western blot - Anti-CD27 antibody (ab175403)All lanes : Anti-CD27 antibody (ab175403) at 1/3000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HL-60 cell lysate
Lane 5 : Mouse thymus tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat AntiRabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 90 secondsBlocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody (ab175403)Paraffin-embedded rat spleen tissue stained for CD27 using ab175403 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-CD27 antibody (ab175403)Immunofluorescence staining of rat thymus tissue stained for CD27 with ab175403 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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Immunocytochemistry/ Immunofluorescence - Anti-CD27 antibody (ab175403)Immunofluorescence staining of Jurkat cells stained for CD27 with ab175403 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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