重组Anti-CD24抗体[EPR3006(N)] (ab179821)
![Western blot - Anti-CD24 antibody [EPR3006(N)] (ab179821)](https://www.abcam.cn/ps/products/179/ab179821/Images/ab179821-208201-anti-cd24-antibody-epr3006n-western-blot.jpg "Western blot - Anti-CD24 antibody [EPR3006(N)] (ab179821)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3006(N)] to CD24
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-CD24抗体[EPR3006(N)]
参阅全部 CD24 一抗 -
描述
兔单克隆抗体[EPR3006(N)] to CD24 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Human fetal brain, SH-SY5Y cell and human oligodendroglioma lysates.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR3006(N) -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab179821于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 8 kDa.
|
|
| IP |
1/10 - 1/100.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 8 kDa. |
|
IP
1/10 - 1/100. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
靶标
-
功能
Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. -
组织特异性
B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells. -
疾病相关
Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface. -
序列相似性
Belongs to the CD24 family. -
翻译后修饰
Extensively O-glycosylated. -
细胞定位
Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 100133941 Human
- Omim: 600074 Human
- SwissProt: P25063 Human
- Unigene: 644105 Human
- Unigene: 721443 Human
-
别名
- CD 24 antibody
- CD24 antibody
- CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
see all
图片
-
Western blot - Anti-CD24 antibody [EPR3006(N)] (ab179821)All lanes : Anti-CD24 antibody [EPR3006(N)] (ab179821) at 1/1000 dilution
Lane 1 : fetal brain lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : human oligodendroglioma lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 8 kDa -
![Immunoprecipitation - Anti-CD24 antibody [EPR3006(N)] (ab179821)](https://www.abcam.cn/ps/products/179/ab179821/Images/ab179821-208202-anti-cd24-antibody-epr3006n-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD24 antibody [EPR3006(N)] (ab179821)Western blot analysis on immunoprecipitation pellet from (1) SH-SY5Y cell lysate or (2) 1X PBS (negative control) labeling CD24 using ab179821 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
-
![Anti-CD24 antibody [EPR3006(N)] (ab179821)](https://www.abcam.cn/ps/products/179/ab179821/Images/ab179821-3-benefits-of-recombinant-antibodies.png)
Anti-CD24 antibody [EPR3006(N)] (ab179821)
数据表及文件
-
SDS download
-
Datasheet download
文献 (16)
ab179821 被引用在 16 文献中.
- Huang L et al. TBX3 stimulates proliferation and stem cell self-renewal in bladder carcinoma. Histol Histopathol 38:65-72 (2023). PubMed: 35856500
- Schloesser D et al. Senescent cells suppress macrophage-mediated corpse removal via upregulation of the CD47-QPCT/L axis. J Cell Biol 222:N/A (2023). PubMed: 36459066
- Xu X et al. Post-translational modification of CDK1-STAT3 signaling by fisetin suppresses pancreatic cancer stem cell properties. Cell Biosci 13:176 (2023). PubMed: 37743465
- Yang Z et al. Crosstalk between heat shock factor 1 and signal transducer and activator of transcription 3 mediated by interleukin-8 autocrine signaling maintains the cancer stem cell phenotype in liver cancer. J Gastroenterol Hepatol 38:138-152 (2023). PubMed: 36300571
- Bai W et al. LINC00589-dominated ceRNA networks regulate multiple chemoresistance and cancer stem cell-like properties in HER2+ breast cancer. NPJ Breast Cancer 8:115 (2022). PubMed: 36309503