重组Anti-CD23抗体[SP23] (ab16702)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] (ab16702)](https://www.abcam.cn/ps/products/16/ab16702/Images/ab16702-5-anti-cd23-antibody-sp23-immunohistochemistry-human-tonsil.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] (ab16702)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP23] to CD23
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD23抗体[SP23]
参阅全部 CD23 一抗 -
描述
兔单克隆抗体[SP23] to CD23 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human CD23 aa 1-250. The exact sequence is proprietary.
Database link: P06734 -
阳性对照
- IHC-P: Human tonsil. Flow Cyt: Jurkat cell lysate. WB: Raji cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
SP23 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16702于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
|
|
| IHC-P |
1/100.
Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
|
| Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. |
|
IHC-P
1/100. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
|
Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). -
序列相似性
Contains 1 C-type lectin domain. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Cell membrane. Secreted. Also exists as a soluble excreted form. - Information by UniProt
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数据库链接
- Entrez Gene: 2208 Human
- Omim: 151445 Human
- SwissProt: P06734 Human
- Unigene: 465778 Human
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别名
- Blast 2 antibody
- BLAST-2 antibody
- Blast2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] (ab16702)
Immunohistochemical analysis of paraffin-embedded Human tonsil labeling CD23 with ab16702 at 1/100 (76 µg/ml). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16702 for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Counterstained with Hematoxylin and mounted with DPX.
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![Flow Cytometry - Anti-CD23 antibody [SP23] (ab16702)](https://www.abcam.cn/ps/products/16/ab16702/Images/ab16702-215233-anti-cd23-antibody-sp23-flow-cytometry.jpg)
Flow Cytometry - Anti-CD23 antibody [SP23] (ab16702)This image was generated using a previous batch manufactured using hybridoma production method.
Flow cytometric analysis of rabbit anti-CD23 (SP23) antibody ab16702 (1/100) in Jurkats (green) compared to negative control of rabbit IgG (blue).
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![Western blot - Anti-CD23 antibody [SP23] (ab16702)](https://www.abcam.cn/ps/products/16/ab16702/Images/ab16702-25557-anti-cd23-antibody-sp23-western-blot.jpg)
Western blot - Anti-CD23 antibody [SP23] (ab16702)Anti-CD23 antibody [SP23] (ab16702) at 1/25 dilution + lysate prepared from Raji cells
Observed band size: 45 kDa why is the actual band size different from the predicted?This image was generated using a previous batch manufactured using hybridoma production method.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab16702 被引用在 5 文献中.
- Hayashi Y et al. Density and maturity of peritumoral tertiary lymphoid structures in oesophageal squamous cell carcinoma predicts patient survival and response to immune checkpoint inhibitors. Br J Cancer 128:2175-2185 (2023). PubMed: 37016103
- Xu W et al. Heterogeneity in tertiary lymphoid structures predicts distinct prognosis and immune microenvironment characterizations of clear cell renal cell carcinoma. J Immunother Cancer 11:N/A (2023). PubMed: 38040418
- Fukano C et al. Establishment of enzyme-linked immunosorbent facilitated antigen binding as a biomarker assay for Japanese cedar pollen allergy immunotherapy. J Pharmacol Sci 140:223-227 (2019). PubMed: 31353210
- Siliņa K et al. A Quantitative Pathology Approach to Analyze the Development of Human Cancer-Associated Tertiary Lymphoid Structures. Methods Mol Biol 1845:71-86 (2018). PubMed: 30141008
- Koletsa T et al. Synchronous Presence of Nasopharyngeal Carcinoma and Marginal Zone (MALT-Type) B-Cell Lymphoma in the Pharynx. Patholog Res Int 2011:340763 (2011). PubMed: 21660262