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重组RabMAb

重组Anti-CD226抗体[EPR23641-137] - BSA and Azide free (ab318184)

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Immunocytochemistry/ Immunofluorescence - Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (ab318184)
  • Flow Cytometry - Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (ab318184)
  • Flow Cytometry - Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (ab318184)
  • Flow Cytometry - Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (ab318184)
  • Flow Cytometry - Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (ab318184)
  • Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (ab318184)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23641-137] to CD226 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-CD226抗体[EPR23641-137] - BSA and Azide free
    参阅全部 CD226 一抗

  • 描述

    兔单克隆抗体[EPR23641-137] to CD226 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, Flow Cytmore details
    不适用于: IHC-P,IP or WB

  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • ICC/IF: Human PBMC cells. Flow Cyt: HEL, Jurkat and Human PBMC cells.

  • 常规说明

    ab318184 is the carrier-free version of ab318183

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab318184于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
说明
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
应用说明
Is unsuitable for IHC-P,IP or WB.

靶标

  • 功能

    Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.

  • 组织特异性

    Expressed by peripheral blood T-lymphocytes.

  • 序列相似性

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • adhesion glycoprotein antibody
      • CD226 antibody
      • CD226 antigen antibody
      • CD226 molecule antibody
      • CD226_HUMAN antibody
      • DNAM 1 antibody
      • DNAM-1 antibody
      • DNAM1 antibody
      • DNAX accessory molecule 1 antibody
      • platelet and T cell activation antigen 1 antibody
      • PTA1 antibody
      • T lineage specific activation antigen 1 antigen antibody
      • TLiSA1 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (ab318184)
      Immunocytochemistry/ Immunofluorescence - Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (ab318184)

      This data was developed using ab318183, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC cells labelling CD226 with ab318183 at 1/100 (5.14 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

      Confocal image showing membranous staining in subsets of human PBMC (shown in green), which is co-localized with CD3 marker. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Anti-human CD3 mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

    • Flow Cytometry - Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (ab318184)
      Flow Cytometry - Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (ab318184)

      This data was developed using ab318183, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of HEL (human erythroleukemia erythroblast) (Right) / K-562 (human chronic myelogenous leukemia lymphoblast) (Left). cells labelling CD226 with ab318183 at 1/5000 dilution (0.1ug) / Magenta compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Gated on viable cells.
      Negative control: K-562 (PMID: 8673704).

    • Flow Cytometry - Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (ab318184)
      Flow Cytometry - Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (ab318184)

      This data was developed using ab318183, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte from peripheral blood) (Right) / MCF7 (human breast adenocarcinoma epithelial cell) (Left). cells labelling CD226 with ab318183 at 1/5000 dilution (0.1ug) / Magenta compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Gated on viable cells.
      Negative control: MCF7 (PMID: 8673704).

    • Flow Cytometry - Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (ab318184)
      Flow Cytometry - Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (ab318184)

      This data was developed using ab318183, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell). cells labelling CD226 with ab318183 at 1/5000 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Cells were co-stained with anti-CD3 conjugated to Alexa Fluor® 647.
      Gated on viable cells.

    • Flow Cytometry - Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (ab318184)
      Flow Cytometry - Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (ab318184)

      This data was developed using ab318183, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell). cells labelling CD226 with ab318183 at 1/5000 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Cells were co-stained with anti-CD19 conjugated to PE/Cy7.The low expression pattern is consistent with what described in the literature. (PMID: 8673704)
      Gated on viable cells.

    • Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (ab318184)
      Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (ab318184)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab318184？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab318184 尚未被引用在任何文献中。

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