Anti-CD22抗体[BLCAM/1795]
Anti-CD22 antibody [BLCAM/1795]
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(1 Publication)
Mouse Monoclonal CD22 antibody. Suitable for ICC, Flow Cyt, WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CD22 aa 50-200.
查看别名
CD22, SIGLEC2, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, Sialic acid-binding Ig-like lectin 2, T-cell surface antigen Leu-14, BL-CAM, Siglec-2
- WB
Supplier Data
Western blot - Anti-CD22 antibody [BLCAM/1795] (AB218340)
All lanes:
Western blot - Anti-CD22 antibody [BLCAM/1795] (ab218340) at 1 µg/mL
Lane 1:
Raji cell lysate
Lane 2:
Ramos cell lysate
Predicted band size: 95 kDa
false
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [BLCAM/1795] (AB218340)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CD22 with ab218340.
- ICC
Supplier Data
Immunocytochemistry - Anti-CD22 antibody [BLCAM/1795] (AB218340)
Immunocytochemistry analysis of paraformaldehyde-fixed Ramos (human Burkitt's lymphoma cell line) cells labeling CD22 with ab218340 followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
- Flow Cyt
Supplier Data
Flow Cytometry - Anti-CD22 antibody [BLCAM/1795] (AB218340)
Flow Cytometry analysis of paraformaldehyde-fixed MOLT-4 (human lymphoblastic leukemia cell line) cells labeling CD22 with ab218340 followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [BLCAM/1795] (AB218340)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human tonsil tissue labeling CD22 with ab218340 at 2 μg/ml dilution followed by conjugation to the secondary antibody.
- Protein Array
Unknown
Protein Array - Anti-CD22 antibody [BLCAM/1795] (AB218340)
ab218340 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14 then the S-score for the binding of that MAb to protein X is equal to 29.
反应性数据
性能和储存信息
形式
纯化工艺
存储溶液
运输条件
推荐的短期储存时间
推荐的短期储存条件
推荐的长期储存条件
分装信息
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD22 acts mainly as a negative regulator of B cell receptor (BCR) signaling. It is part of a complex that interacts with the BCR and other accessory molecules. CD22 helps maintain B cell tolerance by inhibiting overactive signaling and preventing autoimmune responses. By binding to sialic acid-containing glycans CD22 modulates interactions between B cells and other cells aiding in the maintenance of immune homeostasis.
Pathways
CD22 plays a critical role in the BCR signaling pathway and the regulation of phosphatidylinositol 3-kinase (PI3K) signaling. It works together with proteins like CD19 and SHP-1 that are key components of these pathways. By influencing these signaling cascades CD22 contributes to the modulation of B cell activation proliferation and apoptosis ensuring proper functioning of the immune response.
产品实验方案
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靶点信息
文献 (1)
Recent publications for all applications. Explore the full list and refine your search
The Journal of cell biology 222: PubMed36459066
2022
Applications
Unspecified application
Species
Unspecified reactive species
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