Anti-CD22抗体[2H1C4] (ab181771)
Key features and details
- Mouse monoclonal [2H1C4] to CD22
- Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-CD22抗体[2H1C4]
参阅全部 CD22 一抗 -
描述
小鼠单克隆抗体[2H1C4] to CD22 -
宿主
Mouse -
经测试应用
适用于: ICC/IF, Flow Cyt, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human CD22 aa 621-725. expressed in E. Coli.
Sequence:NPPVSHYTWFDWNNQSLPYHSQKLRLEPVKVQHSGAYWCQGTNSVGKGRS PLSTLTVYYSPETIGRRVAVGLGSCLAILILAICGLKLQRRWKRTQSQQG LQENS
Database link: P20273 -
阳性对照
- Human CD22 recombinant protein; HEK293 cell lysate, transfected with CD22 (amino acids 622-725); Mouse L1210, HeLa, HEK293, Jurkat, OCM-1, A432 and mouse NIH 3T3 cell lysates; HeLa cells; Human cervical cancer and cerebellum tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
2H1C4 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181771于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/200 - 1/1000.
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WB |
1/500 - 1/2000. Predicted molecular weight: 95 kDa.
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说明 |
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ICC/IF
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
1/200 - 1/1000. |
WB
1/500 - 1/2000. Predicted molecular weight: 95 kDa. |
靶标
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功能
Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. -
组织特异性
B-lymphocytes. -
序列相似性
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
结构域
Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
翻译后修饰
Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
Phosphorylated on tyrosine residues by LYN. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 933 Human
- Entrez Gene: 12483 Mouse
- Omim: 107266 Human
- SwissProt: P20273 Human
- SwissProt: P35329 Mouse
- Unigene: 579691 Human
- Unigene: 260994 Mouse
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别名
- B cell receptor CD22 precursor antibody
- B lymphocyte cell adhesion molecule antibody
- B-cell receptor CD22 antibody
see all
图片
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Flow cytometric analysis of HeLa cells labeling CD22 with ab181771 at 1/200 dilution (green); negative control (red).
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Anti-CD22 antibody [2H1C4] (ab181771) at 1/500 dilution + Human CD22 recombinant protein
Predicted band size: 95 kDa -
All lanes : Anti-CD22 antibody [2H1C4] (ab181771) at 1/500 dilution
Lane 1 : HEK293 cell lysate, non-transfected
Lane 2 : HEK293 cell lysate, transfected with CD22 (amino acids 622-725)-hIgGFc
Predicted band size: 95 kDa -
All lanes : Anti-CD22 antibody [2H1C4] (ab181771) at 1/500 dilution
Lane 1 : mouse L1210 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HEK293 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : OCM-1 cell lysate
Lane 6 : A431 cell lysate
Lane 7 : mouse NIH 3T3 cell lysate
Predicted band size: 95 kDa -
Immunofluorescent analysis of HeLa cells, labeling CD22 with ab181771 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling CD22 with ab181771 at 1/200 dilution followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling CD22 with ab181771 at 1/200 dilution followed by DAB staining.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab181771 被引用在 1 文献中.
- Frensch M et al. Bacterial lectin BambL acts as a B cell superantigen. Cell Mol Life Sci 78:8165-8186 (2021). PubMed: 34731252