重组Anti-CD21+CD35抗体[EPR27417-508] (ab317494)
![Western blot - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)](https://www.abcam.cn/ps/products/317/ab317494/Images/ab317494-1-anti-cd21cd35-antibody-epr27417-508-western-blot-wehi-231-mouse-b-cell-lymphoma-b-lymphocyte--whole-cell-lysate-20-ug.jpg "Western blot - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR27417-508] to CD21+CD35
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-CD21+CD35抗体[EPR27417-508]
参阅全部 CD21+CD35 一抗 -
描述
兔单克隆抗体[EPR27417-508] to CD21+CD35 -
宿主
Rabbit -
特异性
Unsuitable for human WB
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经测试应用
适用于: IHC-P, WBmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Human
不与反应: Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: WEHI-231, A20, Mouse spleen lysates. IHC-P: Human tonsil, Mouse spleen tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR27417-508 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317494于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| WB |
1/1000.
|
| 说明 |
|---|
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000. |
靶标
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相关性
CD21 also known as complement receptor 2 (CR2), C3d receptor or EBV receptor is a 140 kDa protein. CD21 is a glycosylated type I transmembrane protein consisting of an extracellular face of a series of 15 or 16 CCP domains. CD21 is the receptor for complement components C3d and iC3b as well as the Epstein-Barr virus (EBV) glycoprotein gp350/220. The soluble CD21 (sCD21) was shown to efficiently trigger CD23 signalling pathways in human monocytes. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, sCD21 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders. The CD35 gene encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined. -
细胞定位
Membrane; Single pass type I membrane protein. -
数据库链接
- Entrez Gene: 1378 Human
- Entrez Gene: 1380 Human
- Entrez Gene: 12902 Mouse
- Omim: 120620 Human
- Omim: 120650 Human
- SwissProt: P17927 Human
- SwissProt: P20023 Human
- SwissProt: P19070 Mouse
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别名
- C3 binding protein antibody
- C3b/C4b receptor antibody
- C3BR antibody
see all
图片
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Western blot - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)All lanes : Anti-CD21+CD35 antibody [EPR27417-508] (ab317494) at 1/1000 dilution
Lane 1 : WEHI-231 (mouse B cell lymphoma B lymphocyte ) whole cell lysate
Lane 2 : EL4 (mouse lymphoma T lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 190,250 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: EL4(PMID: 10453011)
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 32907542)
In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.
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![Western blot - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)](https://www.abcam.cn/ps/products/317/ab317494/Images/ab317494-2-anti-cd21cd35-antibody-epr27417-508-western-blot-a20-mouse-reticulum-sarcoma-b-lymphocyte-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)All lanes : Anti-CD21+CD35 antibody [EPR27417-508] (ab317494) at 1/1000 dilution
Lane 1 : A20 (mouse reticulum sarcoma b lymphocyte) whole cell lysate
Lane 2 : EL4.IL-2 (mouse lymphoma T lymphocyte) whole cell lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Mouse thymus tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 190,250 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: thymus, EL4.IL-2 (PMID: 2966205)
The identity of the lower MW band at approximately 37 kDa (in lane 1 and 2) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Exposure time: Lane1-2: 180 seconds, Lane3-4: 15 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)](https://www.abcam.cn/ps/products/317/ab317494/Images/ab317494-3-anti-cd21cd35-antibody-epr27417-508-immunohistochemistry-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD21+CD35 with ab317494 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human tonsil.
The section was incubated with ab317494 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)](https://www.abcam.cn/ps/products/317/ab317494/Images/ab317494-4-anti-cd21cd35-antibody-epr27417-508-immunohistochemistry-mouse-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD21+CD35 with ab317494 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse spleen.
The section was incubated with ab317494 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)](https://www.abcam.cn/ps/products/317/ab317494/Images/ab317494-5-anti-cd21cd35-antibody-epr27417-508-immunohistochemistry-human-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD21+CD35 with ab317494 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human liver.
The section was incubated with ab317494 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)](https://www.abcam.cn/ps/products/317/ab317494/Images/ab317494-6-anti-cd21cd35-antibody-epr27417-508-immunohistochemistry-mouse-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CD21+CD35 with ab317494 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse liver.
The section was incubated with ab317494 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)](https://www.abcam.cn/ps/products/317/ab317494/Images/ab317494-7-Benefits-of-Recombinant-antibodies.jpg)
Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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