重组Anti-CD21+CD35抗体[4B2] (ab243317)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [4B2] to CD21+CD35
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
Related conjugates and formulations
概述
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产品名称
Anti-CD21+CD35抗体[4B2]
参阅全部 CD21+CD35 一抗 -
描述
小鼠单克隆抗体[4B2] to CD21+CD35 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: Flow Cyt,IHC-Fr,IHC-P or IP -
种属反应性
与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is considered to be commercially sensitive.
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阳性对照
- WB: 2.4G2 whole cell lysate. ICC/IF: WEHI-231 and 2.4G2 cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
4B2 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab243317于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.487 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 113 kDa).
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ICC/IF |
Use a concentration of 2.435 µg/ml.
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说明 |
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WB
Use a concentration of 0.487 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 113 kDa). |
ICC/IF
Use a concentration of 2.435 µg/ml. |
靶标
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相关性
CD21 also known as complement receptor 2 (CR2), C3d receptor or EBV receptor is a 140 kDa protein. CD21 is a glycosylated type I transmembrane protein consisting of an extracellular face of a series of 15 or 16 CCP domains. CD21 is the receptor for complement components C3d and iC3b as well as the Epstein-Barr virus (EBV) glycoprotein gp350/220. The soluble CD21 (sCD21) was shown to efficiently trigger CD23 signalling pathways in human monocytes. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, sCD21 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders. The CD35 gene encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined. -
细胞定位
Membrane; Single pass type I membrane protein. -
数据库链接
- Entrez Gene: 12902 Mouse
- Entrez Gene: 289395 Rat
- SwissProt: P19070 Mouse
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别名
- C3 binding protein antibody
- C3b/C4b receptor antibody
- C3BR antibody
see all
图片
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 2.4G2 (Rat B cell lymphoma B lymphocyte) cells labeling CD21 with ab243317 at 2.435µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in 2.4G2 cell line. Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary (red). The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized WEHI-231 (Mouse B cell lymphoma B lymphocyte) or EL4 (Mouse lymphoma T lymphocyte) cells labeling CD21 with ab243317 at 2.435µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and weak cytoplasmic staining in WEHI-231 cell line. Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary (red). The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.
Negative control: EL4 (PMID: 10453011).
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All lanes : Anti-CD21+CD35 antibody [4B2] (ab243317) at 0.487 µg/ml
Lane 1 : EL4 (mouse lymphoma T lymphocyte), whole cell lysate
Lane 2 : 2.4G2 (rat B cell lymphoma B lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG at 1/10000 dilution
Predicted band size: 113 kDa
Observed band size: 155 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Negative control: EL4 (PMID: 10453011).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab243317 被引用在 1 文献中.
- Kulik L et al. A new mouse anti-mouse complement receptor type 2 and 1 (CR2/CR1) monoclonal antibody as a tool to study receptor involvement in chronic models of immune responses and disease. Mol Immunol 63:479-88 (2015). PubMed: 25457881