Anti-CD209B/DC-SIGNR1抗体[ER-TR9] (ab37220)
![Flow Cytometry - Anti-CD209B/DC-SIGNR1 antibody [ER-TR9] (ab37220)](https://www.abcam.cn/ps/products/37/ab37220/Images/ab37220-49754-anti-sign-related-1-antibody-er-tr9-flow-cytometry.jpg "Flow Cytometry - Anti-CD209B/DC-SIGNR1 antibody [ER-TR9] (ab37220)")
Key features and details
- Rat monoclonal [ER-TR9] to CD209B/DC-SIGNR1
- Suitable for: Flow Cyt, IHC-Fr, Blocking
- Reacts with: Mouse
- Isotype: IgM
Related conjugates and formulations
概述
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产品名称
Anti-CD209B/DC-SIGNR1抗体[ER-TR9]
参阅全部 CD209B/DC-SIGNR1 一抗 -
描述
大鼠单克隆抗体[ER-TR9] to CD209B/DC-SIGNR1 -
宿主
Rat -
经测试应用
适用于: Flow Cyt, IHC-Fr, Blockingmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to CD209. Mouse thymic stromal cells
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阳性对照
- Peritoneal macrophages
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
ER-TR9 -
同种型
IgM -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab37220于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 2µg for 106 cells.
ab35768 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr | (2) |
Use at an assay dependent concentration.
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| Blocking |
Use at an assay dependent concentration.
Dilutions have to be made according to the amounts of SIGN-R1 to be inactivated. |
| 说明 |
|---|
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Flow Cyt
Use 2µg for 106 cells. ab35768 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody. |
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IHC-Fr
Use at an assay dependent concentration. |
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Blocking
Use at an assay dependent concentration. Dilutions have to be made according to the amounts of SIGN-R1 to be inactivated. |
靶标
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相关性
SIGN R1 is a specific marker for the identification of macrophage subpopulations present in the marginal zone of spleen (the so-called marginal zone macrophages (MZM)), in the lymph node medulla, and in some strains, in the peritoneal cavity. MZM of the spleen are involved in the clearance of polysaccharides. Mouse SIGN R1 is a C type lectin, like DC SIGN which is expressed on Human dendritic cells (DCs). However, Mmouse SIGN R1 itself is not expressed on DCs. SIGN R1 exists in an aggregated form, resistant to dissociation into monomers upon boiling in SDS under reducing conditions. SIGN R1 mediates the uptake of encapsulated organisms and may be an important mediator for the uptake of microbes in both spleen and lymph node, particularly through the recognition of microbial polysaccharides. -
细胞定位
Single-pass type II membrane protein -
数据库链接
- Entrez Gene: 69165 Mouse
- SwissProt: Q8CJ91 Mouse
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别名
- CD209 antigen like protein B antibody
- CD209b antigen antibody
- DC SIGN related protein 1 antibody
see all
图片
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Flow Cytometry - Anti-CD209B/DC-SIGNR1 antibody [ER-TR9] (ab37220)
Overlay histogram showing SP0/2 cells stained with ab37220 (red line). The cells were fixed with 4% paraformaldehyde (10min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37220, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgM, kappa monoclonal [RTK2118] (ab35768, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SP0/2 cells fixed with 80% methanol (5 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
实验方案
数据表及文件
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Datasheet download
文献 (4)
ab37220 被引用在 4 文献中.
- Ádori M et al. Altered Marginal Zone B Cell Selection in the Absence of I?BNS. J Immunol 200:775-787 (2018). PubMed: 29222168
- Taylor PR et al. The role of SIGNR1 and the beta-glucan receptor (dectin-1) in the nonopsonic recognition of yeast by specific macrophages. J Immunol 172:1157-62 (2004). Blocking, Flow Cyt ; Mouse . PubMed: 14707091
- Kretschmer K et al. The selection of marginal zone B cells differs from that of B-1a cells. J Immunol 171:6495-501 (2003). IHC-Fr ; Mouse . PubMed: 14662849
- Dijkstra CD et al. Marginal zone macrophages identified by a monoclonal antibody: characterization of immuno- and enzyme-histochemical properties and functional capacities. Immunology 55:23-30 (1985). IHC-Fr ; Mouse . PubMed: 3888828
