Anti-CD200 / OX2抗体[MRC OX-2] (ab22353)
![Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX-2] (ab22353)](https://www.abcam.cn/ps/products/22/ab22353/Images/ab22353-2-ab22353Flow1.jpg "Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX-2] (ab22353)")
Key features and details
- Mouse monoclonal [MRC OX-2] to CD200 / OX2
- Suitable for: Flow Cyt
- Reacts with: Rat
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-CD200 / OX2抗体[MRC OX-2]
参阅全部 CD200 / OX2 一抗 -
描述
小鼠单克隆抗体[MRC OX-2] to CD200 / OX2 -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Rat -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt: Lewis rat splenocytes.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified from TCS. -
Primary antibody说明
This antibody is a useful marker for the follicular dendritic cells in lymphoid tissues as it does not label the dendritic (interdigitating) cells in T areas that are Ia antigen positive. -
克隆
单克隆 -
克隆编号
MRC OX-2 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab22353于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use a concentration of 0.2 µg/ml.
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| 说明 |
|---|
|
Flow Cyt
Use a concentration of 0.2 µg/ml. |
靶标
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功能
Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. -
序列相似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 24560 Rat
- SwissProt: P04218 Rat
- Unigene: 7085 Rat
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别名
- Antigen identified by monoclonal antibody MRC OX 2 antibody
- CD200 antibody
- CD200 antigen antibody
see all
图片
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Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX-2] (ab22353)
Lewis rat splenocytes stained with ab22353 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab22353) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab22353 尚未被引用在任何文献中。