AB9475

Anti-CD20 抗体 [L26]

Name: CD20抗体[L26] (ab9475)| Abcam中文官网
Brand: Abcam Limited
SKU: AB9475
Rating: 5 (8 reviews)

Anti-CD20 antibody [L26]

(8 Reviews)

(101 Publications)

Anti-CD20 antibody [L26] (ab9475) is a mouse monoclonal antibody detecting CD20 in IHC-P. Suitable for Human.

- Over 70 publications
- Trusted since 2002

查看别名

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [L26] (AB9475)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [L26] (AB9475)

Formalin-fixed, paraffin-embeded human tonsil tissue stained for CD20 using ab9475 at 1/50 dilution in immunohistochemical analysis. Antigen retrieval with citrate buffer pH 6.0.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

L26

亚型

IgG2a

轻链类型

kappa

不含载体蛋白

反应种属

Human

应用

IHC-P

applications

免疫原

Cell preparation containing MS4A1 protein. The exact immunogen used to generate this antibody is proprietary information.

P11836

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }

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产品详情

What is this antibody validated in?
Anti-CD20 antibody [L26] (ab9475) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Trusted by the scientific community
Anti-CD20 [L26] (ab9475) was first used in a scientific publication in 2002 and has been cited over 70 times in peer-reviewed journals.

Reviewed by scientists
Anti-CD20 [L26] (ab9475) has over 5 independent reviews from customers.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.3 - 7.5 Preservative: 0.1% Sodium azide

运输条件

Blue Ice

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.

Biological function summary

CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.

Pathways

CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.

CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).

See full target information MS4A1

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文献 (101)

Recent publications for all applications. Explore the full list and refine your search

JCO precision oncology 9:e2500404 PubMed40749151

2025

Mucin 16-Directed Therapy in Pediatric Sarcomas: Case Evidence of Ubamatamab Efficacy in Epithelioid Sarcoma and Its Implications for Other Sarcoma Subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Denise M Connolly,Gabriel Revon-Rivière,Rose Chami,Denise Mills,Ailish C Coblentz,Thomas S Uldrick,David A Knorr,Priscila Goncalves,Michael Dobosz,Sumreen Jalal,Sarah Cohen-Gogo,Daniel A Morgenstern

Cancers 17: PubMed40723259

2025

The Prognostic Role of Tertiary Lymphoid Structures and Immune Microenvironment Signatures in Early-Stage -Mutant Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Hsun Hsu,Chia-Chi Hsu,Min-Shu Hsieh,James Chih-Hsin Yang

ACS omega 10:17416-17434 PubMed40352528

2025

Mercury Can Bond to α-Carbon of Curcumin Increasing Stability in Aqueous Medium and Demonstrated Selective Cytotoxicity against Acute Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Sougata Mondal,Upasana Das,Oyendrila Ghosh,Bidisha Maiti,Shuvam Halder,Uttam Pal,Kalyan Kusum Mukherjee,Supratim Ghosh

Oncology letters 29:188 PubMed40007623

2025

Response‑adapted involved site radiation therapy for hepatic marginal zone B‑cell lymphoma: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Shin-Ting Chen,Yu-Guang Chen,Wen-Yen Huang,Cheng-Hsiang Lo

Anticancer research 45:639-650 PubMed39890197

2025

Efficacy of Second-line Nivolumab Tyrosine Kinase Inhibitors for Renal Cell Carcinoma With Bone Metastases.

Applications

Unspecified application

Species

Unspecified reactive species

Gaku Yamamichi,Taigo Kato,Akihiro Yoshimura,Masaru Tani,Yuki Horibe,Yutong Liu,Nesrine Sassi,Yohei Okuda,Toshiki Oka,Toshihiro Uemura,Akinaru Yamamoto,Y U Ishizuya,Takuji Hayashi,Yoshiyuki Yamamoto,Koji Hatano,Atsunari Kawashima,Tetsuya Takao,Kensaku Nishimura,Shingo Takada,Masao Tsujihata,Norio Nonomura

Scientific reports 15:2211 PubMed39820772

2025

B cells enhance IL-1 beta driven invasiveness in triple negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole J Toney,Lynn M Opdenaker,Lisa Frerichs,Shirin R Modarai,Aihui Ma,Holly Archinal,Grace O Ajayi,Jennifer Sims-Mourtada

Therapeutic advances in gastroenterology 17:17562848241309445 PubMed39735350

2024

CD8+ cell dominance in immune checkpoint inhibitor-induced colitis and its heterogeneity across endoscopic features.

Applications

Unspecified application

Species

Unspecified reactive species

Min Kyu Kim,Hye-Nam Son,Seung Wook Hong,Sang Hyoung Park,Dong-Hoon Yang,Byong Duk Ye,Jeong-Sik Byeon,Seung-Jae Myung,Suk-Kyun Yang,Shinkyo Yoon,Sung Wook Hwang

Journal of translational medicine 22:1152 PubMed39731106

2024

Association of PD-1 + Treg/PD-1 + CD8 ratio and tertiary lymphoid structures with prognosis and response in advanced gastric cancer patients receiving preoperative treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Liu,Danhua Xu,Chengbei Zhou,Yiqing Zhong,Haigang Geng,Chen Huang,Yanying Shen,Xiang Xia,Chaojie Wang,Chunchao Zhu,Hui Cao

Cancer science 116:350-366 PubMed39601159

2024

Pan-immune-inflammation value predicts immunotherapy response and reflects local antitumor immune response in rectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qianyu Wang,Wentao Zhong,Yi Xiao,Guole Lin,Junyang Lu,Lai Xu,Guannan Zhang,Aijun Liu,Junfeng Du,Bin Wu

Nature communications 15:7713 PubMed39231979

2024

Single-cell and spatial transcriptome analyses reveal tertiary lymphoid structures linked to tumour progression and immunotherapy response in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Shuang-Yan Ye,Shuai He,Dong-Mei Chi,Xiu-Zhi Wang,Yue-Feng Wen,Dong Ma,Run-Cong Nie,Pu Xiang,You Zhou,Zhao-Hui Ruan,Rou-Jun Peng,Chun-Ling Luo,Pan-Pan Wei,Guo-Wang Lin,Jian Zheng,Qian Cui,Mu-Yan Cai,Jing-Ping Yun,Junchao Dong,Hai-Qiang Mai,Xiaojun Xia,Jin-Xin Bei

View all publications

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