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AB326057

Anti-CD20 antibody [EPR30913-533] - BSA and Azide free (Capture)

Anti-CD20 antibody [EPR30913-533] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal CD20 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

查看别名

CD20, Cd20, Ly-44, Ms4a2, Ms4a1, B-lymphocyte antigen CD20, B-cell differentiation antigen Ly-44, Lymphocyte antigen 44, Membrane-spanning 4-domains subfamily A member 1

2 Images
Sandwich ELISA - Anti-CD20 antibody [EPR30913-533] - BSA and Azide free (Capture) (AB326057)
  • sELISA

Lab

Sandwich ELISA - Anti-CD20 antibody [EPR30913-533] - BSA and Azide free (Capture) (AB326057)

Interpolated concentration of native CD20 was measured at different sample concentrations. Undiluted samples are as follows : A20 cell extract (125 ug/mL) and WEHI-231 cell extract (250 ug/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-CD20 antibody [EPR30913-533] - BSA and Azide free (Capture) (AB326057)
  • sELISA

Lab

Sandwich ELISA - Anti-CD20 antibody [EPR30913-533] - BSA and Azide free (Capture) (AB326057)

Sandwich ELISA with the capture antibody ab326057 dilution at 2 µg/mL and detector antibody ab326058 dilution at 0.5 µg/mL after biotin conjugation.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR30913-533

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

产品详情

ab326057 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse CD20. The recommended pair for sandwich ELISA is:

Capture: ab326057, Mouse CD20 Capture Antibody (unconjugated)

Detector: ab326058, Mouse CD20 Detector Antibody (unconjugated)

The reference range value is 3.9-250 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.
See full target information Ms4a1

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