Anti-CD20抗体(ab27093)
Key features and details
- Rabbit polyclonal to CD20
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CD20抗体
参阅全部 CD20 一抗 -
描述
兔多克隆抗体to CD20 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human CD20 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P11836 -
常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab27093 is an affinity purified IgG. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab27093于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
|
|
| IHC-P |
1/300. Antigen retrieval is not essential but may optimise staining.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa. |
|
IHC-P
1/300. Antigen retrieval is not essential but may optimise staining. |
靶标
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功能
This protein may be involved in the regulation of B-cell activation and proliferation. -
组织特异性
Expressed on B-cells. -
疾病相关
Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
序列相似性
Belongs to the MS4A family. -
翻译后修饰
Phosphorylated. Might be functionally regulated by protein kinase(s). -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 931 Human
- Omim: 112210 Human
- SwissProt: P11836 Human
- Unigene: 712553 Human
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别名
- APY antibody
- ATOPY antibody
- B lymphocyte antigen CD20 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (ab27093)Formalin fixed, paraffin embedded human tonsil tissue slide stained with anti CD20 antibody (ab27093).
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Western blot - Anti-CD20 antibody (ab27093)Anti-CD20 antibody (ab27093) at 1/25 dilution + Raji cell lysate
Predicted band size: 33 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab27093 被引用在 10 文献中.
- Kosenda K et al. Histological Characteristics of Conjunctiva-Associated Lymphoid Tissue in Young and Adult Holstein Cattle. Animals (Basel) 13:N/A (2023). PubMed: 38003099
- Sirivisoot S et al. Evaluation of 41 single nucleotide polymorphisms in canine diffuse large B-cell lymphomas using MassARRAY. Sci Rep 12:5120 (2022). PubMed: 35332215
- Wei ZW et al. Immune-infiltration based signature as a novel prognostic biomarker in gastrointestinal stromal tumour. EBioMedicine 57:102850 (2020). PubMed: 32574962
- Webb GM et al. The human IL-15 superagonist ALT-803 directs SIV-specific CD8+T cells into B-cell follicles. Blood Adv 2:76-84 (2018). IHC-P ; Rhesus monkey . PubMed: 29365313
- Stahl EC et al. Evaluation of the host immune response to decellularized lung scaffolds derived from a-Gal knockout pigs in a non-human primate model. Biomaterials 187:93-104 (2018). PubMed: 30312852
- Miles B et al. Follicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection. PLoS Pathog 12:e1005924 (2016). IHC (PFA fixed) ; Rhesus monkey . PubMed: 27716848
- Miles B et al. Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection. Nat Commun 6:8608 (2015). PubMed: 26482032
- Bojarczuk K et al. B-cell receptor pathway inhibitors affect CD20 levels and impair antitumor activity of anti-CD20 monoclonal antibodies. Leukemia N/A:N/A (2014). Human . PubMed: 24492323
- Connick E et al. Compartmentalization of simian immunodeficiency virus replication within secondary lymphoid tissues of rhesus macaques is linked to disease stage and inversely related to localization of virus-specific CTL. J Immunol 193:5613-25 (2014). PubMed: 25362178
- Moll NM et al. Imaging correlates of leukocyte accumulation and CXCR4/CXCL12 in multiple sclerosis. Arch Neurol 66:44-53 (2009). IHC-Fr ; Human . PubMed: 19139298