Anti-CD2抗体[TS218] - BSA and Azide free (ab175287)
Key features and details
- Mouse monoclonal [TS218] to CD2 - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-CD2抗体[TS218] - BSA and Azide free
参阅全部 CD2 一抗 -
描述
小鼠单克隆抗体[TS218] to CD2 - BSA and Azide free -
宿主
Mouse -
特异性
Does not work with Rat in Western Blot applications -
经测试应用
适用于: WB, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Full length protein corresponding to Human CD2. Native protein
Database link: P06729 -
阳性对照
- Human PBMC cells; Jurkat cells, Ramos, A-431, Raji.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Constituent: 100% PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purified IgG from mouse ascites fluid. -
克隆
单克隆 -
克隆编号
TS218 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175287于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 39 kDa.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 39 kDa. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function. -
序列相似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 914 Human
- Omim: 186990 Human
- SwissProt: P06729 Human
- Unigene: 523500 Human
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别名
- CD 2 antibody
- CD2 antibody
- CD2 antigen (p50), sheep red blood cell receptor antibody
see all
图片
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All lanes : Anti-CD2 antibody [TS218] - BSA and Azide free (ab175287) at 2 µg/ml
Lane 1 : Ramos
Lane 2 : A-431
Lane 3 : K562
Lane 4 : Raji
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution
Predicted band size: 39 kDa -
Flow cytometric analysis of Jurkat cells labeling CD2 with ab175287 at a dilution of 0.5 µg/test (green), compared to an isotype control (blue). A Dylight 488-conjugated goat anti-mouse IgG (H+L) was used as the secondary antibody.
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Flow cytometric analysis of PBMC cells labeling CD2 with ab175287 at a dilution of 0.5 µg/test (green), compared to an isotype control (blue). A Dylight 488-conjugated goat anti-mouse IgG (H+L) was used as the secondary antibody.
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Flow cytometric analysis of PBMC cells labeling CD2 with ab175287 at a dilution of 0.5 µg/test (green), compared to an isotype control (blue). A Dylight 488-conjugated goat anti-mouse IgG (H+L) was used as the secondary antibody.
数据表及文件
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Datasheet download
文献 (0)
ab175287 尚未被引用在任何文献中。