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重组RabMAb

重组Anti-CD2抗体[SP304] - BSA and Azide free (ab272017)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
  • Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP304] to CD2 - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-CD2抗体[SP304] - BSA and Azide free
    参阅全部 CD2 一抗

  • 描述

    兔单克隆抗体[SP304] to CD2 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human tonsil, thymus, colon, stomach, stomach adenocarcinoma, colon adenocarcinoma, lung squamous cell carcinoma, lung adenocarcinoma, uterus and cervical squamous cell carcinoma tissue.

  • 常规说明

    ab272017 is the carrier-free version of ab227698.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272017于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration.

Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Incubate with primary antibody for 10 minutes at room temperature.

 
说明
IHC-P
Use at an assay dependent concentration.

Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Incubate with primary antibody for 10 minutes at room temperature.

 

靶标

  • 功能

    CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

  • 序列相似性

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CD 2 antibody
      • CD2 antibody
      • CD2 antigen (p50), sheep red blood cell receptor antibody
      • CD2 antigen antibody
      • CD2 molecule antibody
      • CD2_HUMAN antibody
      • Erythrocyte receptor antibody
      • FLJ46032 antibody
      • LFA-2 antibody
      • LFA-3 receptor antibody
      • LFA2 antibody
      • LFA3 receptor antibody
      • Ly-37 antibody
      • Lymphocyte function antigen 2 antibody
      • lymphocyte-function antigen-2 antibody
      • OTTHUMP00000024366 antibody
      • Rosette receptor antibody
      • Sheep erythrocyte receptor antibody
      • SRBC antibody
      • T cell surface antigen CD2 antibody
      • T-cell surface antigen CD2 antibody
      • T-cell surface antigen T11/Leu-5 antibody
      • T-lymphocyte surface CD2 antigen antibody
      • T11 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)

      Immunohistochemistry analysis of paraffin-embedded human tonsil tissue sections labelling CD2 with ab227698 at 1/100 dilution. The section was incubated with ab227698 for 10 mins at room temperature. LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
      Positive staining on the human tonsil tissue, performed on a Leica Biosystems BOND® RX instrument.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)

      Formalin-fixed, paraffin-embedded human thymus tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)

      Formalin-fixed, paraffin-embedded human colon tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)

      Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)

      Formalin-fixed, paraffin-embedded human uterus tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)

      Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)

      Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)

      Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)

      Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)

      Formalin-fixed, paraffin-embedded human stomach tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).
    • Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)
      Anti-CD2 antibody [SP304] - BSA and Azide free (ab272017)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab272017？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab272017 尚未被引用在任何文献中。

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com