重组Anti-CD2抗体[EPR6451] (ab131276)
![Flow Cytometry (Intracellular) - Anti-CD2 antibody [EPR6451] (ab131276)](https://www.abcam.cn/ps/products/131/ab131276/Images/ab131276-506241-anti-cd2-antibody-epr6451-flowcytometry-jurkat-human.jpg "Flow Cytometry (Intracellular) - Anti-CD2 antibody [EPR6451] (ab131276)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6451] to CD2
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD2抗体[EPR6451]
参阅全部 CD2 一抗 -
描述
兔单克隆抗体[EPR6451] to CD2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IP, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human CD2 aa 200-350. The exact sequence is proprietary.
Database link: P06729 -
阳性对照
- WB: Human Jurkat cell lysates. IHC: Human tonsil, colon and spleen tissue. IP: Jurkat lysate. Flow cyto: Jurkat (Human T cell leukemia T lymphocyte) cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6451 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131276于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/2500.
|
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
|
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| IP |
1/10 - 1/100.
|
|
| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/2500. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa. |
|
IP
1/10 - 1/100. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function. -
序列相似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 914 Human
- Omim: 186990 Human
- SwissProt: P06729 Human
- Unigene: 523500 Human
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别名
- CD 2 antibody
- CD2 antibody
- CD2 antigen (p50), sheep red blood cell receptor antibody
see all
图片
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Flow Cytometry (Intracellular) - Anti-CD2 antibody [EPR6451] (ab131276)
Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD2 with purified ab131276 at 1:20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as a isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)](https://www.abcam.cn/ps/products/131/ab131276/Images/ab131276-506240-anti-cd2-antibody-epr6451-immunohistochemistry-spleen-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)Immunohistochemical analysis of Paraffin-embedded sections Human spleen tissue labelling CD2 with ab131276 at 1/1000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on Human spleen tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)](https://www.abcam.cn/ps/products/131/ab131276/Images/ab131276-506239-anti-cd2-antibody-epr6451-immunohistochemistry-colon-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)Immunohistochemical analysis of Paraffin-embedded sections Human colon tissue labelling CD2 with ab131276 at 1/1000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on Human colon tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)](https://www.abcam.cn/ps/products/131/ab131276/Images/ab131276-506238-anti-cd2-antibody-epr6451-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)Immunohistochemical analysis of Paraffin-embedded sections Human tonsil tissue labelling CD2 with ab131276 at 1/1000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on Human tonsil tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Western blot - Anti-CD2 antibody [EPR6451] (ab131276)](https://www.abcam.cn/ps/products/131/ab131276/Images/ab131276-506237-anti-cd2-antibody-epr6451-westernblot-human.jpg)
Western blot - Anti-CD2 antibody [EPR6451] (ab131276)Anti-CD2 antibody [EPR6451] (ab131276) at 1/1000 dilution + Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 45-58 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 10510361).
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![Immunoprecipitation - Anti-CD2 antibody [EPR6451] (ab131276)](https://www.abcam.cn/ps/products/131/ab131276/Images/ab131276-10-anti-cd2-antibody-epr6451-immunoprecipitation-jurkat-human.jpg)
Immunoprecipitation - Anti-CD2 antibody [EPR6451] (ab131276)CD2 was immunoprecipitated from 0.35 mg Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10 µg with 131276 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10 µg
Lane 2: ab131276 IP in Jurkat whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab131276 in Jurkat whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Anti-CD2 antibody [EPR6451] (ab131276)](https://www.abcam.cn/ps/products/131/ab131276/Images/ab131276-8-benefits-of-recombinant-antibodies.png)
Anti-CD2 antibody [EPR6451] (ab131276)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab131276 被引用在 3 文献中.
- Qiu C & Du G Loss of LEF-1 expression as a diagnostic indicator for extranodal NK/T-cell lymphoma: An immunohistochemical study of 88 cases. Eur J Haematol 109:513-518 (2022). PubMed: 35871391
- Santucci-Pereira J et al. Genomic signature of parity in the breast of premenopausal women. Breast Cancer Res 21:46 (2019). PubMed: 30922380
- Companioni Nápoles O et al. SCHLAFEN 5 expression correlates with intestinal metaplasia that progresses to gastric cancer. J Gastroenterol 52:39-49 (2017). PubMed: 27032393