重组Anti-CD1d抗体[EPR22526-9] - BSA and Azide free

• Flow Cyt

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Flow Cytometry - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (AB256357)

Flow cytometric analysis of K562 (human chronic myelogenous leukemia lymphoblast, Left) / Jurkat (human T cell leukemia T lymphocyte, Right) cells labeling CD1d with ab256344 at 1/600 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

Negative control : K562 (PMID : 21037579).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256344).

• Flow Cyt

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Flow Cytometry - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (AB256357)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD1d with ab256344 at 1/600 dilution (0.1μg) (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150097) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab256344 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor^® 647. Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256344).

• IP

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Immunoprecipitation - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (AB256357)

CD1d was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate with ab256344 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256344 at 1/1000 dilution (0.6 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate (10μg)
Lane 2 : ab256344 IP in Jurkat whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab256344 in Jurkat whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256344).

All lanes:

Immunoprecipitation - Anti-CD1d antibody [EPR22526-9] (<a href='/products/primary-antibodies/cd1d-antibody-epr22526-9-ab256344'>ab256344</a>)

Predicted band size: 38 kDa

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