AB254464

重组Anti-CD1d抗体[EPR22526-72] - BSA and Azide free

Anti-CD1d antibody [EPR22526-72] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal CD1D antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

查看别名

CD1d, Antigen-presenting glycoprotein CD1d, R3G1, CD1D

1 Images

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Immunoprecipitation - Anti-CD1d antibody [EPR22526-72] - BSA and Azide free (AB254464)

CD1d was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole lysate with ab229903 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229903 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1 : Jurkat whole cell lysate 10 μg (Input).

Lane 2 : ab229903 IP in Jurkat whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab229903 in Jurkat whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229903).

All lanes:

Immunoprecipitation - Anti-CD1d antibody [EPR22526-72] (<a href='/products/primary-antibodies/cd1d-antibody-epr22526-72-ab229903'>ab229903</a>)

Predicted band size: 38 kDa

Observed band size: 48 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-CD1d antibody [EPR22526-72]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22526-72

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

ab254464 is the carrier-free version of ab229903.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD1d is a non-classical major histocompatibility complex (MHC) class I-like molecule also known as the CD1d molecule. This protein has a mass of approximately 37 kDa and plays an important role in the presentation of lipid antigens to T cells. CD1d is mainly expressed on the surface of antigen-presenting cells including dendritic cells B cells and epithelial cells in the intestine. The expression pattern highlights its relevance in immune responses at mucosal surfaces.

Biological function summary

CD1d functions by presenting lipid and glycolipid antigens to a specialized subset of T cells called invariant Natural Killer T (iNKT) cells. CD1d forms a complex with these antigens in the endosomal compartment before migrating to the cell surface. This interaction is critical for activating iNKT cells which then produce cytokines that influence both innate and adaptive immune responses. It is distinct in its role compared to classical MHC molecules in antigen presentation.

Pathways

CD1d's role in antigen presentation influences numerous immune signaling pathways. Its interaction with iNKT cells is part of the broader immune regulatory network impacting the Th1/Th2 cytokine balance and contributing to immune surveillance. CD1d works alongside proteins involved in these pathways such as CD1b and CD1c which also present lipids to T cells highlighting their involvement in maintaining immune system homeostasis.

CD1d has been linked to autoimmune diseases and cancers. Within autoimmune disorders the interaction between CD1d and iNKT cells affects disease pathogenesis possibly through cytokine dysregulation. In some cancers CD1d expression changes can disrupt normal immune function allowing tumors to progress. Through these diseases CD1d interacts with proteins such as Nor32 and Nor3 impacting how immune cells recognize and respond to diseased or cancerous cells.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.

See full target information CD1D

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1508559 PubMed40078987

2025

Single-cell RNA-sequencing reveals cellular heterogeneity and immune microenvironment characteristics between ocular adnexal mucosa-associated lymphoid lymphoma and IgG4-related ophthalmic disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Yang,Yujiao Wang,Xuelian Jin,Weimin He

View all publications

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重组Anti-CD1d抗体[EPR22526-72] - BSA and Azide free