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使用敲除细胞株进行验证重组RabMAb

重组Anti-CD1a抗体[EP3622] (ab108309)

Submit a review Q&A (2)References (5)
Western blot - Anti-CD1a antibody [EP3622] (ab108309)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EP3622] (ab108309)
  • Western blot - Anti-CD1a antibody [EP3622] (ab108309)
  • Anti-CD1a antibody [EP3622] (ab108309)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3622] to CD1a
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Carrier Free

概述

  • 产品名称

    Anti-CD1a抗体[EP3622]
    参阅全部 CD1a 一抗

  • 描述

    兔单克隆抗体[EP3622] to CD1a

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Human fetal thymus lysate, human T-78 cell lysate, human skin tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • 解离常数（KD）

    KD = 1.30 x 10 -11 M
    Learn more about KD

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EP3622

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab108309于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/250 dilution.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/250 dilution.
应用说明
Is unsuitable for Flow Cyt.

靶标

  • 功能

    Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

  • 组织特异性

    Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.

  • 序列相似性

    Contains 1 Ig-like (immunoglobulin-like) domain.

  • 细胞定位

    Cell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes. Localizes to cell surface lipid rafts.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CD 1a antibody
      • CD1 antibody
      • CD1a antibody
      • CD1A Antigen antibody
      • CD1A antigen, a polypeptide antibody
      • CD1a molecule antibody
      • CD1A_HUMAN antibody
      • cluster of differentiation 1 A antibody
      • cortical thymocyte antigen CD1A antibody
      • differentiation antigen CD1 alpha 3 antibody
      • epidermal dendritic cell marker CD1a antibody
      • FCB 6 antibody
      • FCB6 antibody
      • HTA 1 antibody
      • HTA1 antibody
      • hTa1 thymocyte antigen antibody
      • OTTHUMP00000018907 antibody
      • R 4 antibody
      • R4 antibody
      • T 6 antibody
      • T-cell surface antigen T6/Leu-6 antibody
      • T-cell surface glycoprotein CD1a antibody
      • T6 antibody
      see all

    图片

    • Western blot - Anti-CD1a antibody [EP3622] (ab108309)
      Western blot - Anti-CD1a antibody [EP3622] (ab108309)
      All lanes : Anti-CD1a antibody [EP3622] (ab108309) at 1/1000 dilution

      Lane 1 : Wild-type Jurkat cell lysate
      Lane 2 : CD1A knockout Jurkat cell lysate
      Lane 3 : MOLT-4 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 37 kDa
      Observed band size: 45-50 kDa why is the actual band size different from the predicted?



      False colour image of Western blot: Anti-CD1a antibody [EP3622] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108309 was shown to bind specifically to CD1a. A band was observed at 45-50 kDa in wild-type Jurkat cell lysates with no signal observed at this size in CD1A knockout cell line ab274926 (knockout cell lysate ab274984). To generate this image, wild-type and CD1A knockout Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EP3622] (ab108309)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EP3622] (ab108309)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thymoma tissue sections labelling CD1a with purified ab108309 at 1/1000 dilution (0.14 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    • Western blot - Anti-CD1a antibody [EP3622] (ab108309)
      Western blot - Anti-CD1a antibody [EP3622] (ab108309)
      Anti-CD1a antibody [EP3622] (ab108309) at 1/1000 dilution (Purified) + Human thymus lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 37 kDa
      Observed band size: 49 kDa why is the actual band size different from the predicted?



      The molecular weight observed is consistent with what has been described in the literature (PMID: 18178838).

    • Anti-CD1a antibody [EP3622] (ab108309)
      Anti-CD1a antibody [EP3622] (ab108309)

    文献 (5)

    发表研究结果有使用 ab108309？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab108309 被引用在 5 文献中.

    • Hu YQ & Zhang JZ A Comparison for Type 2 Cytokines and Lesional Inflammatory Infiltrations in Bullous Pemphigoid and Atopic Dermatitis. Clin Cosmet Investig Dermatol 15:2313-2321 (2022). PubMed: 36325102
    • Bolognesi MM  et al. Multiplex Staining by Sequential Immunostaining and Antibody Removal on Routine Tissue Sections. J Histochem Cytochem 65:431-444 (2017). PubMed: 28692376
    • Nguyen N  et al. Tumor infiltrating lymphocytes and survival in patients with head and neck squamous cell carcinoma. Head Neck 38:1074-84 (2016). Human . PubMed: 26879675
    • Tam J  et al. Reconstitution of full-thickness skin by microcolumn grafting. J Tissue Eng Regen Med N/A:N/A (2016). IHC-P ; Human . PubMed: 27296503
    • Perelygina L  et al. Rubella persistence in epidermal keratinocytes and granuloma M2 macrophages in patients with primary immunodeficiencies. J Allergy Clin Immunol 138:1436-1439.e11 (2016). IF ; Human . PubMed: 27613149

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    Searching for rabbit specific
    - anti CD3
    - anti FOXP3
    - anti CD4
    - anti CD8
    - anti CD1A
    antibodies

    Read More
    Answer

    Thank you very much for your phone call.

    The best antibodies we can recommend are as follows. Please note that the recommendations are based on immunogen sequence only we currently do not hold any rabbit specific data.

    Anti CD3 : ab16669; I can provide Abreview image discount code and will also provide Abpromise guarantee on use for this ab.

    Anti FOXP3: ab20034, ab22510 are best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

    Anti CD4: ab61131, ab101530 and ab108329, are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

    anti CD8: ab108343, ab123630 and ab86733, are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

    Anti CD1A : ab76531, ab108309 and ab123133 are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

    Please also note that most of these antibodies are raised in Rabbit so there will be high back ground staining. Our recommendations to reduce the back ground staining are

    - Use preadsorbed anti body e.g. ab7132, ab7083, ab

    - Use (Fab)2 fragments antibodies e.g. ab6112, ab102296

    - Incubate sections with affinity pure Fab fragment anti rabbit antibody before applying primary antibody

    Please check the full details by clicking the links;

    https://www.abcam.com/index.html?pageconfig=resource&rid=12722
    https://www.abcam.com/index.html?pageconfig=resource&rid=12825
    https://www.abcam.com/index.html?pageconfig=resource&rid=11465

    The abreview discount code details can be found by clicking following link;

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

    Could you please select the antibodies you are interested in, I will then be happy in creating a discount code for each one of these.

    I will look forward to hearing from you soon.

    Read More

    Question

    I am looking for hCD1a antibody for western blot usage, I came across the two antibodies in your product catalogue: Anti-CD1a antibody [EP3622] (ab108309), Anti-CD1a antibody [EP3091] (ab76531) However, I failed to find the targeting epitope information as to the two antibodies while I am interested to know the recognition area of them? Could you help me with it? Thanks in advance. Best Regards,

    Read More
    Answer

    Thank you for contacting us. The immunogen of ab108309 is from amino acid XXXXXXXX of human CD1a protein. The immunogen of ab76531 is from amino acid XXXXXX of human CD1a. Click to check the compatible products: https://www.abcam.com/index.html?pageconfig=productmap&cl=918 https://www.abcam.com/index.html?pageconfig=resource&rid=13148 https://www.abcam.com/index.html?pageconfig=resource&rid=12906 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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