Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
使用敲除细胞株进行验证RabMAb

Anti-CD1a抗体[EP3091] (ab76531)

Submit a review Q&A (4)References (2)
Western blot - Anti-CD1a antibody [EP3091] (ab76531)
  • Western blot - Anti-CD1a antibody [EP3091] (ab76531)

Key features and details

  • Rabbit monoclonal [EP3091] to CD1a
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-CD1a抗体[EP3091]
    参阅全部 CD1a 一抗

  • 描述

    兔单克隆抗体[EP3091] to CD1a

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details
    不适用于: Flow Cyt,IHC-P or IP

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: MOLT4, K562, fetal thymus lysates, CD1A knockout Jurkat cell lysate and HeLa cell lysate

  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Tissue culture supernatant

  • 克隆

    单克隆

  • 克隆编号

    EP3091

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab76531于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
应用说明
Is unsuitable for Flow Cyt,IHC-P or IP.

靶标

  • 功能

    Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

  • 组织特异性

    Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.

  • 序列相似性

    Contains 1 Ig-like (immunoglobulin-like) domain.

  • 细胞定位

    Cell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes. Localizes to cell surface lipid rafts.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CD 1a antibody
      • CD1 antibody
      • CD1a antibody
      • CD1A Antigen antibody
      • CD1A antigen, a polypeptide antibody
      • CD1a molecule antibody
      • CD1A_HUMAN antibody
      • cluster of differentiation 1 A antibody
      • cortical thymocyte antigen CD1A antibody
      • differentiation antigen CD1 alpha 3 antibody
      • epidermal dendritic cell marker CD1a antibody
      • FCB 6 antibody
      • FCB6 antibody
      • HTA 1 antibody
      • HTA1 antibody
      • hTa1 thymocyte antigen antibody
      • OTTHUMP00000018907 antibody
      • R 4 antibody
      • R4 antibody
      • T 6 antibody
      • T-cell surface antigen T6/Leu-6 antibody
      • T-cell surface glycoprotein CD1a antibody
      • T6 antibody
      see all

    图片

    • Western blot - Anti-CD1a antibody [EP3091] (ab76531)
      Western blot - Anti-CD1a antibody [EP3091] (ab76531)
      All lanes : Anti-CD1a antibody [EP3091] (ab76531) at 1/1000 dilution

      Lane 1 : Wild-type Jurkat cell lysate
      Lane 2 : CD1A knockout Jurkat cell lysate
      Lane 3 : MOLT-4 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L preabsorbed (ab216773) and Goat anti-Mouse IgG H&L preabsorbed (ab216776) at 1/20000 dilution

      Performed under reducing conditions.

      Predicted band size: 37 kDa
      Observed band size: 45-50 kDa why is the actual band size different from the predicted?



      False colour image of Western blot: Anti-CD1a antibody [EP3091] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab76531 was shown to bind specifically to CD1a.

      A band was observed at 45-50 kDa in wild-type Jurkat cell lysates with no signal observed at this size in CD1A knockout cell line ab274926 (knockout cell lysate ab274984). To generate this image, wild-type and CD1A knockout Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

      Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

    • Western blot - Anti-CD1a antibody [EP3091] (ab76531)
      Western blot - Anti-CD1a antibody [EP3091] (ab76531)
      All lanes : Anti-CD1a antibody [EP3091] (ab76531) at 1/5000 dilution

      Lane 1 : MOLT4 lysate
      Lane 2 : K562 lysate
      Lane 3 : Fetal thymus lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 37 kDa
      Observed band size: 47 kDa why is the actual band size different from the predicted?

    文献 (2)

    发表研究结果有使用 ab76531？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab76531 被引用在 2 文献中.

    • Gatti E Monitoring MHC Ubiquitination by MARCH Ubiquitin Ligases. Methods Mol Biol 1988:259-270 (2019). PubMed: 31147945
    • Mahiddine K  et al. CD99 isoforms regulate CD1a expression in human monocyte-derived DCs through ATF-2/CREB-1 phosphorylation. Eur J Immunol 46:1460-71 (2016). PubMed: 27094031

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-4 of 4 Abreviews or Q&A

    Question

    IHC-Fr, no signal, however, antibodies are not suitable for frozen section according to ds.
    Protocol tips?
    Alternatives?

    Read More
    Answer

    Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

    Unseres Wissens nach wurde ab123133 Anti-CD1a antibody bisher noch nicht in IHC-Fr getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab123133 abdecken, und gegen eine erneute Bestellung eines weiteren primären Antikörpers von uns verrechnet werden.

    Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

    1.) Bestätigen Sie mir bitte, dass Sie ab123133 in IHC-Fr testen möchten.

    2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

    3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

    4.) Testen Sie den Antikörper in IHC-Fr.

    5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
    Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
    https://www.abcam.com/abreviews

    6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können einen anderen primären Antikörper zu dem gleichen Preis wie ab123133 bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer von ab123133bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

    Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

    Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
    https://www.abcam.com/collaborationdiscount

    Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

    Read More

    Question

    Searching for rabbit specific
    - anti CD3
    - anti FOXP3
    - anti CD4
    - anti CD8
    - anti CD1A
    antibodies

    Read More
    Answer

    Thank you very much for your phone call.

    The best antibodies we can recommend are as follows. Please note that the recommendations are based on immunogen sequence only we currently do not hold any rabbit specific data.

    Anti CD3 : ab16669; I can provide Abreview image discount code and will also provide Abpromise guarantee on use for this ab.

    Anti FOXP3: ab20034, ab22510 are best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

    Anti CD4: ab61131, ab101530 and ab108329, are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

    anti CD8: ab108343, ab123630 and ab86733, are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

    Anti CD1A : ab76531, ab108309 and ab123133 are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

    Please also note that most of these antibodies are raised in Rabbit so there will be high back ground staining. Our recommendations to reduce the back ground staining are

    - Use preadsorbed anti body e.g. ab7132, ab7083, ab

    - Use (Fab)2 fragments antibodies e.g. ab6112, ab102296

    - Incubate sections with affinity pure Fab fragment anti rabbit antibody before applying primary antibody

    Please check the full details by clicking the links;

    https://www.abcam.com/index.html?pageconfig=resource&rid=12722
    https://www.abcam.com/index.html?pageconfig=resource&rid=12825
    https://www.abcam.com/index.html?pageconfig=resource&rid=11465

    The abreview discount code details can be found by clicking following link;

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

    Could you please select the antibodies you are interested in, I will then be happy in creating a discount code for each one of these.

    I will look forward to hearing from you soon.

    Read More

    Question

    I am looking for hCD1a antibody for western blot usage, I came across the two antibodies in your product catalogue: Anti-CD1a antibody [EP3622] (ab108309), Anti-CD1a antibody [EP3091] (ab76531) However, I failed to find the targeting epitope information as to the two antibodies while I am interested to know the recognition area of them? Could you help me with it? Thanks in advance. Best Regards,

    Read More
    Answer

    Thank you for contacting us. The immunogen of ab108309 is from amino acid XXXXXXXX of human CD1a protein. The immunogen of ab76531 is from amino acid XXXXXX of human CD1a. Click to check the compatible products: https://www.abcam.com/index.html?pageconfig=productmap&cl=918 https://www.abcam.com/index.html?pageconfig=resource&rid=13148 https://www.abcam.com/index.html?pageconfig=resource&rid=12906 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Regarding your product - Anti-CD1a antibody [EP3091] (ab76531) You state that this product also react with mouse. As far as I know mice don’t express this protein. Kind regards

    Read More
    Answer

    Thanks for your contact regarding ab76531. After reviewing this enquiry, we have removed mouse species from the datasheet. We apologize for any inconvenience caused and thank you for bringing this matter to our attention.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com