AB25177

Anti-CD19抗体[MB19-1]

Anti-CD19 antibody [MB19-1]

Lab Essentials
了解详情

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(5 Publications)

Mouse Monoclonal CD19 antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 5 publications.

查看别名

CD19, B-lymphocyte antigen CD19, Differentiation antigen CD19, Cd19

1 Images

Flow Cytometry - Anti-CD19 antibody [MB19-1] (AB25177)

Flow Cyt

Lab

Flow Cytometry - Anti-CD19 antibody [MB19-1] (AB25177)

C57BL6 mouse splenocytes were incubated with Mouse IgA kappa monoclonal [S107] (ab37322) (left) or anti-CD19 [MB19-1] antibody (ab25177) (right) at 1ug/ml for 30 min at 4+°C. The secondary antibody Goat Anti-Mouse IgA alpha chain (FITC) was used at 1/200 dilution for 30 min at 4+°C. The cells were simultaneously stained with anti-CD19 APC (10ug/ml).

Acquisition of >30,000 total events were collected. Gating strategy - live lymphocytes

不同偶联物与剂型 (1)

578 PE

PE Anti-CD19 antibody [MB19-1]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

MB19-1

亚型

IgA

不含载体蛋白

反应种属

Mouse

应用

Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-5 µg/mL", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-iga-kappa-s107-isotype-control-ab37322'>ab37322</a> - Mouse monoclonal IgA, is suitable for use as an isotype control with this antibody.</p>" } } }

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产品详情

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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性能和储存信息

形式

Liquid

纯化说明

Diafiltration.

存储溶液

Preservative: 0.02% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD19 is a transmembrane protein also known as B4 or B-lymphocyte antigen CD19 with an approximate molecular weight of 95 kDa. It is expressed on the surface of B cells throughout their development from pro-B cells to mature B cells. CD19 functions mechanically as a coreceptor enhancing the sensitivity of B cell receptor (BCR) signaling. This protein plays a significant role in promoting the activation and proliferation of B cells by lowering the threshold for antigen receptor engagement.

Biological function summary

CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.

Pathways

The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.

CD19 is connected to B cell malignancies and autoimmune diseases. B cell acute lymphoblastic leukemia (ALL) often shows aberrant CD19 expression making it a valuable target for therapeutic interventions like anti-CD19 monoclonal antibodies. Additionally overexpression or mutations of CD19 may contribute to autoimmune diseases by disrupting normal B cell regulation. CD19's connection with proteins like CD22 and CD20 in these pathological contexts highlights its relevance in developing targeted therapies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (By similarity). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed : 12387743, PubMed : 20101619, PubMed : 9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed : 7542548, PubMed : 7543183, PubMed : 9317126). Required for normal differentiation of B-1 cells (PubMed : 12387743, PubMed : 7542548, PubMed : 7543183). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed : 12387743, PubMed : 7542548, PubMed : 9317126). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed : 12387743, PubMed : 7542548, PubMed : 7543183).

See full target information Cd19

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Clinical cancer research : an official journal of 24:6015-6027 PubMed30262507

2018

A Designer Cross-reactive DNA Immunotherapeutic Vaccine that Targets Multiple MAGE-A Family Members Simultaneously for Cancer Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth K Duperret,Shujing Liu,Megan Paik,Aspen Trautz,Regina Stoltz,Xiaoming Liu,Kan Ze,Alfredo Perales-Puchalt,Charles Reed,Jian Yan,Xiaowei Xu,David B Weiner

The Journal of experimental medicine 186:1923-31 PubMed9382890

1998

CD19-regulated signaling thresholds control peripheral tolerance and autoantibody production in B lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

M Inaoki,S Sato,B C Weintraub,C C Goodnow,T F Tedder

Proceedings of the National Academy of Sciences of the United States of America 94:13158-62 PubMed9371816

1997

CD19 and CD22 expression reciprocally regulates tyrosine phosphorylation of Vav protein during B lymphocyte signaling.

Applications

Unspecified application

Species

Unspecified reactive species

S Sato,P J Jansen,T F Tedder

Journal of immunology (Baltimore, Md. : 1950) 159:3278-87 PubMed9317126

1997

Regulation of B lymphocyte development and activation by the CD19/CD21/CD81/Leu 13 complex requires the cytoplasmic domain of CD19.

Applications

Unspecified application

Species

Unspecified reactive species

S Sato,A S Miller,M C Howard,T F Tedder

Immunity 5:551-62 PubMed8986715

1996

CD22 is both a positive and negative regulator of B lymphocyte antigen receptor signal transduction: altered signaling in CD22-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

S Sato,A S Miller,M Inaoki,C B Bock,P J Jansen,M L Tang,T F Tedder

View all publications

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