重组Anti-CD19抗体[EPR5906] (ab134114)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5906] to CD19
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IHC-Fr
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD19抗体[EPR5906]
参阅全部 CD19 一抗 -
描述
兔单克隆抗体[EPR5906] to CD19 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IHC-Frmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human CD19 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: P15391 -
阳性对照
- WB: Namalwa, Daudi and Ramos cell lysates; human tonsil tissue lysate. IHC-P: Human tonsil, diffuse large B-cell lymphoma, B-cell chronic lymphocytic leukaemia and spleen tissue. IHC (Frozen sections) - Human tonsil. ICC/IF: Raji cells. Flow Cyt (intra): Raji cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5906 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- HRP Anti-CD19 antibody [EPR5906] (ab195896)
- Alexa Fluor® 488 Anti-CD19 antibody [EPR5906] (ab196468)
- Alexa Fluor® 647 Anti-CD19 antibody [EPR5906] (ab196515)
- Anti-CD19 antibody [EPR5906] - Low endotoxin, Azide free (ab215382)
- Anti-CD19 antibody [EPR5906] - BSA and Azide free (ab271904)
- Alexa Fluor® 555 Anti-CD19 antibody [EPR5906] (ab274888)
- APC Anti-CD19 antibody [EPR5906] (ab317484)
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134114于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 61 kDa).
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IHC-P | (2) |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
ICC/IF |
1/500 - 1/1000.
|
|
IHC-Fr |
Use a concentration of 1 µg/ml.
|
说明 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 61 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/500 - 1/1000. |
IHC-Fr
Use a concentration of 1 µg/ml. |
靶标
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功能
Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. -
疾病相关
Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
序列相似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
翻译后修饰
Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 930 Human
- Omim: 107265 Human
- SwissProt: P15391 Human
- Unigene: 652262 Human
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别名
- Antibody deficiency due to defect in CD19 antibody
- Antibody deficiency due to defect in CD19, included antibody
- AW495831 antibody
see all
图片
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Intracellular Flow Cytometry analysis of Raji cells (Human Burkitt's lymphoma B lymphocyte)labelling CD19 with ab134114 at 1/1000 dilution, 1.186µg/ml (red). Cells were fixed with 4% paraformaldehyde, permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000.
Isotype control (black) - Rabbit monoclonal IgG (ab172730)
Unlabeled control (blue) - Unlabelled cells
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR5906] (ab134114)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD19 with unpurified ab134114 at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunocytochemistry/Immunofluorescence analysis of Raji cells labelling CD19 with purified ab134114 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
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Lanes 1-3 : Anti-CD19 antibody [EPR5906] (ab134114) at 1/10000 dilution
Lane 4 : Anti-CD19 antibody [EPR5906] (ab134114) at 1/2000 dilution
Lane 1 : NAMALWA (Human Burkitt's lymphoma) whole cell lysate)
Lane 2 : Human tonsil tissue lysate
Lane 3 : Jurkat (Human acute T cell leukemia)whole cell lysate
Lane 4 : RAMOS (Human Burkitt's lymphoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Exposure time:
Lane 1-3: 3 minutes
Lane 4: 30 secondsJurkat is a CD19 null cell line according to the paper (PMID: 19147785).
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All lanes : Anti-CD19 antibody [EPR5906] (ab134114) at 1/5000 dilution (purified)
Lane 1 : Namalwa whole cell lysate
Lane 2 : Daudi whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 61 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR5906] (ab134114)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD19 with purified ab134114 at a dilution of 1/500. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR5906] (ab134114)
Negative tissue: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling CD19 with purified ab134114 at a dilution of 1/500. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR5906] (ab134114)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human diffuse large B-cell lymphoma tissue labelling CD19 with unpurified ab134114.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR5906] (ab134114)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CD19 with unpurified ab134114.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR5906] (ab134114)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human B-cell chronic lymphocytic leukaemia tissue labelling CD19 with unpurified ab134114.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR5906] (ab134114)
Immunohistochemical analysis of paraffin embedded human skeletal muscle tissue using unpurified ab134114 showing negative staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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IHC image of ab134114 staining CD19 in human tonsil frozen tissue sections, performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab134114 at 1µg/ml for 30 mins at room temperature and detected using an HRP conjugated compact polymer system (Bond™ Polymer Refine Detection). DAB was used as the chromogen. Positive membrane staining was seen in human tonsil. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
IHC image of ab134114 staining CD19 in human heart frozen tissue sections, performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab134114 at 1µg/ml for 30 mins at room temperature and detected using an HRP conjugated compact polymer system (Bond™ Polymer Refine Detection). DAB was used as the chromogen. No staining in human heart (negative tissue) was observed. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (54)
ab134114 被引用在 54 文献中.
- Ci W et al. Characteristics of B cells and immunoglobulin profile in Takayasu's arteritis. Clin Exp Rheumatol 41:870-878 (2023). PubMed: 36533979
- Quigley LT et al. Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry. STAR Protoc 4:101961 (2023). PubMed: 36633948
- Subtil B et al. Dendritic cell phenotype and function in a 3D co-culture model of patient-derived metastatic colorectal cancer organoids. Front Immunol 14:1105244 (2023). PubMed: 36761758
- Wang Y et al. Muramyl dipeptide CD10 monoclonal antibody immunoconjugates inhibited acute leukemia in nude mice. Biosci Rep 43:N/A (2023). PubMed: 37039042
- He M et al. Intratumoral tertiary lymphoid structure (TLS) maturation is influenced by draining lymph nodes of lung cancer. J Immunother Cancer 11:N/A (2023). PubMed: 37072348