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Anti-CD19抗体[6D5] (ab25232)

Reviews (3)Q&A (3)References (22)
Flow Cytometry - Anti-CD19 antibody [6D5] (ab25232)

    Key features and details

    • Rat monoclonal [6D5] to CD19
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Isotype: IgG2a

    Conjugates logo Related conjugates and formulations

    APC Cy5 ® PE/Cy5.5® PE/Cy5® PE/Cy7®

    概述

    • 产品名称

      Anti-CD19抗体[6D5]
      参阅全部 CD19 一抗

    • 描述

      大鼠单克隆抗体[6D5] to CD19

    • 宿主

      Rat

    • 经测试应用

      适用于: Flow Cytmore details

    • 种属反应性

      与反应: Mouse

    • 免疫原

      Tissue, cells or virus corresponding to Mouse CD19. Mouse CD19-expressing K562 human erythroleukemia cells

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab25232于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    Use at an assay dependent concentration.

    ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
    说明
    Flow Cyt
    Use at an assay dependent concentration.

    ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

    靶标

    • 功能

      Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

    • 疾病相关

      Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.

    • 序列相似性

      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

    • 翻译后修饰

      Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.

    • 细胞定位

      Membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Antibody deficiency due to defect in CD19 antibody
        • Antibody deficiency due to defect in CD19, included antibody
        • AW495831 antibody
        • B lymphocyte antigen CD19 antibody
        • B lymphocyte surface antigen B4 antibody
        • B-lymphocyte antigen CD19 antibody
        • B-lymphocyte surface antigen B4 antibody
        • B4 antibody
        • CD19 antibody
        • CD19 antigen antibody
        • CD19 molecule antibody
        • Cd19 protein antibody
        • CD19_HUMAN antibody
        • CVID3 antibody
        • Differentiation antigen CD19 antibody
        • Leu 12 antibody
        • Leu-12 antibody
        • Leu12 antibody
        • MGC109570 antibody
        • MGC12802 antibody
        • T-cell surface antigen Leu-12 antibody
        see all

      图片

      • Flow Cytometry - Anti-CD19 antibody [6D5] (ab25232)
        Flow Cytometry - Anti-CD19 antibody [6D5] (ab25232)

        Flow Cytometry analysis of BALB/c mouse splenocyte cells labeling CD19 with ab25232 at 1 μg/106 cells dilution, followed by Rat Anti-Mouse CD3ε-FITC then by Mouse Anti-Rat IgG2a-PE.

      文献 (22)

      发表研究结果有使用 ab25232？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab25232 被引用在 22 文献中.

      • Rokan A  et al. Gut-derived Endotoxin-TLR4 Signaling Drives MYC-Ig Translocation to Promote Lymphoproliferation through c-JUN and STAT3 Activation. Mol Cancer Res 21:155-169 (2023). PubMed: 36287175
      • Belhoul-Fakir H  et al. Injury to the tunica media initiates atherogenesis in the presence of hyperlipidemia. Front Cardiovasc Med 10:1152124 (2023). PubMed: 37063951
      • Ramos A  et al. Leukemia-intrinsic determinants of CAR-T response revealed by iterative in vivo genome-wide CRISPR screening. Nat Commun 14:8048 (2023). PubMed: 38052854
      • Ukita M  et al. CXCL13-producing CD4+ T cells accumulate in the early phase of tertiary lymphoid structures in ovarian cancer. JCI Insight 7:N/A (2022). PubMed: 35552285
      • Giannoccaro MP  et al. Behaviour and neuropathology in mice injected with human contactin-associated protein 2 antibodies. Brain 142:2000-2012 (2019). PubMed: 31079141
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-6 of 6 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD19 antibody [6D5]

       Inconclusive
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (kidney)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citric acid
      Permeabilization
      No
      Specification
      kidney
      Blocking step
      Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
      Fixative
      Masked holm
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Apr 14 2020

      Immunohistochemistry (Frozen sections) abreview for Anti-CD19 antibody [6D5]

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Ileal - Peyer's Patch)
      Permeabilization
      No
      Specification
      Ileal - Peyer's Patch
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Jack Robertson

      Verified customer

      提交于 Oct 04 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD19 antibody [6D5]

       Poor
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Lung)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: 10 mM Citrate buffer
      Permeabilization
      No
      Specification
      Lung
      Blocking step
      Serum as blocking agent for 1 hour(s) and 5 minute(s) · Concentration: 5% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Nov 17 2015

      Question

      Do you have/know where one can obtain a purified unconjugated agonistic monoclonal/polyclonal Ab to human CD19?
      Thank you.

      Read More
      Answer

      We have one unconjugated antibody ab25232 which unfortunately hasn't been tested in functional assays though it has similar clone number then the tested conjugated ones.

      In case you are interested in buying and trying this antibody then I would encourage you to participate in our 100% Abtrial discount programme - a free product can be claimed in exchange of review for this antibody. Please contact me for code if you accept our offer.

      More details about the offer can be found at the following link;

      https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

      Read More

      Question

      Could you suggest antibody conjugated to Red dyes (PE)?

      Read More
      Answer

      Thank you for contacting us.

      Please check the following antibodies;


      https://www.abcam.com/cd19-antibody-6d5-pe-cy5-5-ab25548.html

      https://www.abcam.com/cd19-antibody-mb19-1-pe-cy5-5-ab25384.html

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Question

      Product code: 25232

      Inquiry: Hi, I wanted to know if the antibody ab25232 had been tested for use in IHC-P on mouse tissue? If not could you recommend an antibody that would identify the B-cell population in FFPE mouse tisse sections? Thanks Fiona

      Read More
      Answer

      Thank you very much for your interest in ab25232.
      To our knowledge, ab25232 has not been tested in IHC-P yet.
      We do offer the following antibody that is tested and guaranteed for mouse and IHC-P and might be suitable for your purpose of identifying B cells:
      ab64100b (Anti-CD45R antibody) also know as B220
      https://www.abcam.com/CD45R-antibody-RA3-6B2-ab64100.html https://www.abcam.com/CD45R-antibody-RA3-6B2-ab64100.html.
      If you prefer to use CD19, I can offer a discount off a future purchase if you buy ab25232 now, test it in IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody or the value off your next order.
      If you are interested in this offer, please follow these steps:
      1. Reply to this e-mail to let me know that you would like to proceed and test ab25232 in IHC-P. I will then send a discount code. This code must be issued before purchasing ab25232 so please wait for my reply before ordering.
      2. Purchase ab25232 either by phone, fax, or online (www.abcam.com).
      3. Test it in IHC-P.
      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any product ordered and the discount code is valid for 4 months after issue.
      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab25232 turns out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted.
      Please let me know if you have any questions about this offer and I would be happy to help you further.
      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com