重组Anti-CD18 (phospho S745)抗体[EP1288Y] (ab52920)

Vielen Dank für Ihre Email.

Beide Nummern (1213063 und GR31039-4) beschreiben dasselbe Lot.

Ich kann bestätigen, dass die Konzentration für dieses Lot 1.283 mg/mL ist.

ich hoffe, diese Information ist hilfreich und verbleibe

Vielen Dank für Ihren Anruf.

Ich freue mich nun die Konzentration von Lot 1213063 bestätigen zu können:

1.283 mg/mL.

Ich hoffe, dies wird Ihnen beim Einstellen der Isotypkontrolle weiterhelfen. Bitte beachten Sie, dass ein Kaninchen monoklonaler Antikörper ist.

Thank you for contacting us.

I advise not buying ab52920 if you want to stain all CD18. The antibody is raised against a phosphorylated site on CD18, and will only stain CD18 that is phosphorylated at that site, not total CD18.

The choices of anti- total CD18 antibodies that are known to work in IHC on paraffin-embedded tissues are few. Your best choice of what we have is ab657, a mouse monoclonal that is known to work for IHC on paraffin-embedded tissue. The application had been missing from the datasheet until now. I have asked the laboratory that validated the antibody for this IHC to send their data.

There is no IF data, to our knowledge. It has been validated for flow cytometry, but on live, unfixed cells. We do not know how it will perform on PFA-fixed or methanol-fixed cells.

Please do not hesitate to contact us if you need any more advice or information.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number . To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Thank you for contacting Abcam.

For ab63388 you get 100 µg at 1mg/ml. For ab52920 you get 100ul butthe concentration varies slightly from batch to batch and so if you could let me know the lot number that you currently have, I will be happy to find out the concentration for you.

Please let me know if there is anything else I can help you with.

Thank you for contacting us. ab63388 hasn't been tested in WB so we can’t confirm the suitability of this antibody due to lack of tested data. However this does not mean this cannot be used in WB this only means we have not tested it. If you are interested in testing this ab in WB thenmay need toconsider our collaboration discount code offers. Please click on the link below; https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

The ab52920 can be used in Western Blot, Immunohistochemistry (paraffin embedded tissue sections, immunoprecipitation and Immunocytochemistry / Immunofluorescence. I hope this will help!

Thank you for contacting us. Could you let us know the phosphorylated amino acid you are interested in? The integrin beta 2 shows phosphorylation at Serine 745, 756 and at Threonine 758, 759 and 760. The antibodies we have are ab52920 and ab63388 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Click to check out the compatible secondary antibodies and IHC kits: https://www.abcam.com/index.html?pageconfig=productmap&cl=918 https://www.abcam.com/index.html?pageconfig=catalogkits&intProductTypeID=4