为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Beta

Anti-CD18抗体[MEM-48] (ab657)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (8)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Flow Cytometry - Anti-CD18 antibody [MEM-48] (ab657)

    Key features and details

    • Mouse monoclonal [MEM-48] to CD18
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 700 APC Biotin FITC PE

    你可能会感兴趣

    蛋白
    Product image
    Recombinant Human CD18 protein (ab112311)
    一抗
    FITC Anti-CD19 antibody [CB19] (ab1167)
    一抗
    Anti-CD11+CD18 antibody [24] (ab13219)

    查看更多相关产品

    概述

    • 产品名称

      Anti-CD18抗体[MEM-48]
      参阅全部 CD18 一抗
    • 描述

      小鼠单克隆抗体[MEM-48] to CD18
    • 宿主

      Mouse
    • 特异性

      Human CD18 antigen. This antibody recognizes all three leucocyte integrins.
    • 经测试应用

      适用于: Flow Cytmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Tissue, cells or virus corresponding to Human CD18. Leucocytes of a patient suffering from a LGL-type leukaemia

    • 常规说明

      This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 纯度

      Protein A purified
    • 纯化说明

      Purified from TCS. Purity >95% by SDS-PAGE.
    • 克隆

      单克隆
    • 克隆编号

      MEM-48
    • 同种型

      IgG1
    • 研究领域

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cell Adhesion
      • Integrins
      • Beta
      • Immunology
      • Cell Type Markers
      • CD
      • Adhesion
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • NK Cell Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Dendritic Cell Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Mast Cell Lineage

    相关产品

    • Alternative Versions

      • FITC Anti-CD18 antibody [MEM-48], prediluted (ab18238)
      • Biotin Anti-CD18 antibody [MEM-48] (ab28093)
      • APC Anti-CD18 antibody [MEM-48], prediluted (ab40914)
      • Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free (ab46700)
      • PE Anti-CD18 antibody [MEM-48] (ab49921)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
      • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    • Recombinant Protein

      • Recombinant Human CD18 protein (ab112311)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab657于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt (1)
    Use 0.1-1µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    说明
    Flow Cyt
    Use 0.1-1µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    靶标

    • 功能

      Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
    • 疾病相关

      Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
    • 序列相似性

      Belongs to the integrin beta chain family.
      Contains 1 VWFA domain.
    • 翻译后修饰

      Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
    • 细胞定位

      Membrane.
    • Target information above from: UniProt accession P05107 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 3689 Human
      • Omim: 600065 Human
      • SwissProt: P05107 Human
      • Unigene: 375957 Human
      • 别名

        • 95 subunit beta antibody
        • Beta 2 (antigen CD18 (p95) antibody
        • CD 18 antibody
        • CD18 antibody
        • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
        • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
        • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
        • Complement component 3 receptor 3 and 4 subunit antibody
        • Complement receptor C3 beta subunit antibody
        • Complement receptor C3 subunit beta antibody
        • Integrin beta 2 antibody
        • Integrin beta chain beta 2 antibody
        • Integrin beta-2 (ITGB2) antibody
        • Integrin beta-2 antibody
        • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
        • ITB2_HUMAN antibody
        • ITGB2 antibody
        • LAD antibody
        • LCAMB antibody
        • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
        • Leukocyte cell adhesion molecule CD18 antibody
        • LFA 1 antibody
        • LFA1 antibody
        • Lymphocyte function associated antigen 1 (LFA1) antibody
        • Lymphocyte function associated antigen 1 antibody
        • MAC 1 antibody
        • MAC1 antibody
        • Macrophage antigen 1 (mac1) beta antibody
        • MF17 antibody
        • MFI7 antibody
        • OTTHUMP00000115278 antibody
        • OTTHUMP00000115279 antibody
        • OTTHUMP00000115280 antibody
        • OTTHUMP00000115281 antibody
        • OTTHUMP00000115282 antibody
        see all

      图片

      • Flow Cytometry - Anti-CD18 antibody [MEM-48] (ab657)
        Flow Cytometry - Anti-CD18 antibody [MEM-48] (ab657)

        Human peripheral blood lymphocytes stained with ab657 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22ºC. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37ºC. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4ºC. Cells were then incubated with the antibody (ab657, 1μg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

      实验方案

      • Flow cytometry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (8)

      发表研究结果有使用 ab657?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab657 被引用在 8 文献中.

      • Nice JB  et al. Aggregatibacter actinomycetemcomitans Leukotoxin Is Delivered to Host Cells in an LFA-1-Indepdendent Manner When Associated with Outer Membrane Vesicles. Toxins (Basel) 10:N/A (2018). PubMed: 30322160
      • Laufer JM  et al. CCR7 Is Recruited to the Immunological Synapse, Acts as Co-stimulatory Molecule and Drives LFA-1 Clustering for Efficient T Cell Adhesion Through ZAP70. Front Immunol 9:3115 (2018). PubMed: 30692994
      • Asadi A  et al. Characterization of Antibodies to Products of Proinsulin Processing Using Immunofluorescence Staining of Pancreas in Multiple Species. J Histochem Cytochem 63:646-62 (2015). PubMed: 26216140
      • Xiang Y  et al. Analysis on the relevance of asthma susceptibility with the alteration of integrin ß 4 expression. PLoS One 9:e95533 (2014). WB ; Human . PubMed: 24740264
      • Giese S & Marsh M Tetherin can restrict cell-free and cell-cell transmission of HIV from primary macrophages to T cells. PLoS Pathog 10:e1004189 (2014). ICC/IF . PubMed: 24991932
      • Evani SJ  et al. Monocytes mediate metastatic breast tumor cell adhesion to endothelium under flow. FASEB J 27:3017-29 (2013). Human . PubMed: 23616566
      • Drbal K  et al. Human leukocytes contain a large pool of free forms of CD18. Biochem Biophys Res Commun 275:295-9 (2000). PubMed: 10964661
      • Bazil V  et al. Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein. Folia Biol (Praha) 36:41-50 (1990). PubMed: 1971601

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-2 of 2 Abreviews or Q&A

      Flow Cytometry abreview for Anti-CD18 antibody [MEM-48]

      Good
      Abreviews
      Abreviews
      Application
      Flow Cytometry
      Sample
      Human Cell lysate - whole cell (HEK293 cells overexpressing cd18/cd11b)
      Specification
      HEK293 cells overexpressing cd18/cd11b
      Permeabilization
      No
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Aug 18 2005

      Question

      When these antibodies were tested in Western blotting, what cell line was used for characterization?

      Read More

      Abcam community

      Verified customer

      Asked on Jul 01 2005

      Answer

      Thank you for your enquiry. These two antibodies were characterized using HPB-ALL or Jurkat T cells. Triplet of zones on NON-REDUCING lysates is usually observed (78-96 kDa). If you have any additional questins, please contact us again.

      Read More

      Abcam Scientific Support

      回复于 Jul 07 2005

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      通过邮件收到学术资源和促销信息 注册
      研究类别(A-Z)
      • 肿瘤研究
      • 心血管研究
      • 细胞生物学
      • 发育生物学
      • 染色质及细胞核信号转导研究
      • 免疫学
      • 代谢
      • 微生物学
      • 神经生物学
      • 信号转导研究
      • 干细胞研究
      产品种类(A-Z)
      • 一抗
      • 二抗
      • 生化试剂
      • 亚类对照
      • 流式细胞仪多色选择器
      • 试剂盒
      • 内参对照
      • 细胞裂解液
      • 多肽
      • 蛋白酶
      • 组织切片
      • 蛋白标签及细胞标记物
      • 相关试剂及工具
      帮助与支持
      • 技术支持
      • 技术FAQ
      • 实验步骤与建议
      • 购买FAQ
      • RabMAb 产品
      • 培训课程
      • 按靶标浏览
      企业
      • 企业资讯
      • 投资者关系
      • 公司新闻
      • 招贤纳士
      • 关于我们
      活动通知
      • 商品展览
      • 会议
      全球网站
      • abcam.com
      • abcam.co.jp
      QR code for Weechat

      微信扫码 关注我们

      沪ICP备14046373号-1

      沪公网安备 31011502007413号

      • 销售条款
      • 网站使用条款
      • Cookie政策
      • 隐私权政策
      • 法律
      © 1998-2023 艾博抗(上海)贸易有限公司版权所有