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Anti-CD18抗体[MEM-148] (ab8220)

Reviews (1)Q&A (3)References (13)
Flow Cytometry - Anti-CD18 antibody [MEM-148] (ab8220)

    Key features and details

    • Mouse monoclonal [MEM-148] to CD18
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    APC PE

    概述

    • 产品名称

      Anti-CD18抗体[MEM-148]
      参阅全部 CD18 一抗

    • 描述

      小鼠单克隆抗体[MEM-148] to CD18

    • 宿主

      Mouse

    • 特异性

      Recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1.

    • 经测试应用

      适用于: Flow Cytmore details

    • 种属反应性

      与反应: Human
      不与反应: Pig, Monkey

    • 免疫原

      Tissue, cells or virus corresponding to Human CD18. Peripheral blood mononuclear cells

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab8220于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    Use 0.1µg for 106 cells.

    The antibody is excellent marker of activated myeloid cells.

     

     

     

     

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     
    说明
    Flow Cyt
    Use 0.1µg for 106 cells.

    The antibody is excellent marker of activated myeloid cells.

     

     

     

     

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    靶标

    • 功能

      Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.

    • 疾病相关

      Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.

    • 序列相似性

      Belongs to the integrin beta chain family.
      Contains 1 VWFA domain.

    • 翻译后修饰

      Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.

    • 细胞定位

      Membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • 95 subunit beta antibody
        • Beta 2 (antigen CD18 (p95) antibody
        • CD 18 antibody
        • CD18 antibody
        • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
        • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
        • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
        • Complement component 3 receptor 3 and 4 subunit antibody
        • Complement receptor C3 beta subunit antibody
        • Complement receptor C3 subunit beta antibody
        • Integrin beta 2 antibody
        • Integrin beta chain beta 2 antibody
        • Integrin beta-2 (ITGB2) antibody
        • Integrin beta-2 antibody
        • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
        • ITB2_HUMAN antibody
        • ITGB2 antibody
        • LAD antibody
        • LCAMB antibody
        • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
        • Leukocyte cell adhesion molecule CD18 antibody
        • LFA 1 antibody
        • LFA1 antibody
        • Lymphocyte function associated antigen 1 (LFA1) antibody
        • Lymphocyte function associated antigen 1 antibody
        • MAC 1 antibody
        • MAC1 antibody
        • Macrophage antigen 1 (mac1) beta antibody
        • MF17 antibody
        • MFI7 antibody
        • OTTHUMP00000115278 antibody
        • OTTHUMP00000115279 antibody
        • OTTHUMP00000115280 antibody
        • OTTHUMP00000115281 antibody
        • OTTHUMP00000115282 antibody
        see all

      图片

      • Flow Cytometry - Anti-CD18 antibody [MEM-148] (ab8220)
        Flow Cytometry - Anti-CD18 antibody [MEM-148] (ab8220)

        Human peripheral blood lymphocytes stained with ab8220 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab8220, 0.1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

      文献 (13)

      发表研究结果有使用 ab8220？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab8220 被引用在 13 文献中.

      • Baganha F  et al. Phosphorylcholine Monoclonal Antibody Therapy Decreases Intraplaque Angiogenesis and Intraplaque Hemorrhage in Murine Vein Grafts. Int J Mol Sci 23:N/A (2022). PubMed: 36362449
      • Baganha F  et al. Atorvastatin pleiotropically decreases intraplaque angiogenesis and intraplaque haemorrhage by inhibiting ANGPT2 release and VE-Cadherin internalization. Angiogenesis 24:567-581 (2021). PubMed: 33550461
      • Li D  et al. ß-1,3-Glucan/CR3/SYK pathway-dependent LC3B-II accumulation enhanced the fungicidal activity in human neutrophils. J Microbiol N/A:N/A (2019). PubMed: 30721460
      • Trentini A  et al. Hydroxyethyl Starch 130/0.4 Binds to Neutrophils Impairing Their Chemotaxis through a Mac-1 Dependent Interaction. Int J Mol Sci 20:N/A (2019). PubMed: 30769810
      • Vogel ME  et al. Bilirubin Prevents Atherosclerotic Lesion Formation in Low-Density Lipoprotein Receptor-Deficient Mice by Inhibiting Endothelial VCAM-1 and ICAM-1 Signaling. J Am Heart Assoc 6:N/A (2017). PubMed: 28365565
      View all Publications for this product

      客户评价及客户问答

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      提交评价 提交问题

      1-4 of 4 Abreviews or Q&A

      Western blot abreview for Anti-CD18 antibody [MEM-148]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Hep-3B,HLF)
      Gel Running Conditions
      Non-reduced Denaturing
      Loading amount
      20 µg
      Treatment
      25 µM BSO for 24hrs
      Specification
      Hep-3B,HLF
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Dr. Chengpeng Yu

      Verified customer

      提交于 Nov 26 2020

      Question

      ab8220 detects multiple bands in WB

      Read More
      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***.

      To check the status of the order please contact our Customer Service team and reference this number.

      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

      Read More

      Question

      Dear technical service, I would appreciate information concerning the number of cells (neutrophils) and levels of antibodies that you would recommend using for the following antibodies: ab8220 primary antibody ab6785 secondary ab7002-500 secondary We will further test saturation for our cells in our laboratory but would appreciate your initial advice.

      Read More
      Answer

      There is a citation from the published methodology (Drbal et al. Blood 98(5), pp1561-1566, 2001): mAb used in paper: MEM-148 (ab8220) MEM-48 (standard anti CD18, #11-216) and other mAbs supplied by Abcam Cell were stained with mAbs (20 ug/ml) for 30 min on ice and washed in HBSS (Hanks balanced salt solution) containing 0.2% gelatin and 0.1% NaN3 without Ca++ and Mg++ followed by fluorescein labeled antimouse Ig. Propidium iodide (0.1 ug/ml) and LDS-751 (0.2 ug/ml) were added prior to measurement on FACS in a standard 3-color setup. At least 10000 viable nucleated cells (PI- , LDS-751+) were collected for each sample. Leucocyte populations were resolved on the basis of their scatter properties.

      Read More

      Question

      Technical Services, Does CD18-ab8220 also recognize the high affinity state of Mac-1(CD11b/CD18)? Your product data sheet states that it recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high affinity state of LFA-1 (CD11a/CD18).

      Read More
      Answer

      It is not clear whether ab8220 recognizes the high-affinity (activated) conformation also on Mac-1, we have only indirect indications that it is the case but no definitive unambiguous data

      Read More

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