Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-CD16+CD32抗体[EPR23501-203] - BSA and Azide free (ab275095)

Submit a review Submit a question
Western blot - Anti-CD16+CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
  • Flow Cytometry - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
  • Flow Cytometry - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD16+CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
  • Immunoprecipitation - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
  • Western blot - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
  • Immunoprecipitation - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
  • Western blot - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
  • Anti-CD16+CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23501-203] to CD16+CD32 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Unconjugated

概述

  • 产品名称

    Anti-CD16+CD32抗体[EPR23501-203] - BSA and Azide free
    参阅全部 CD16+CD32 一抗

  • 描述

    兔单克隆抗体[EPR23501-203] to CD16+CD32 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
    不适用于: IHC-Fr

  • 种属反应性

    与反应: Mouse

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: J774A.1 whole cell lysates; Mouse spleen and liver tissue lysates; His-tagged mouse CD16 recombinant protein; His-tagged mouse CD32 recombinant protein. IHC-P: Mouse spleen, liver and colon carcinoma tissue. ICC/IF: J774A.1 cells. Flow cyt: J774A.1 and mouse splenocyte cells. IP: J774A.1 whole cell lysate; Mouse spleen tissue lysate.

  • 常规说明

    ab275095 is the carrier-free version of ab223200.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab275095于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Detects a band of approximately 40-60 kDa.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Detects a band of approximately 40-60 kDa.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
应用说明
Is unsuitable for IHC-Fr.

靶标

  • 相关性

    Function: Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. Tissue specificity: Found on monocytes, neutrophils and eosinophil platelets. Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. PTM: Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.

  • 细胞定位

    CD32: Type I membrane protein. CD16: Attached to the membrane by a GPI anchor. Exists also as a soluble receptor, produced by a proteolytic cleavage.

  • 数据库链接

    • 别名

      • CD16a antibody
      • CD16b antibody
      • CD32 antibody
      • CD32A antibody
      • CD32B antibody
      • CDw32 antibody
      • Fc fragment of IgG low affinity IIa receptor antibody
      • Fc fragment of IgG low affinity IIb receptor antibody
      • Fc fragment of IgG low affinity IIIa receptor antibody
      • Fc fragment of IgG low affinity IIIb receptor antibody
      • Fc gamma receptor III A antibody
      • Fc gamma receptor IIIb antibody
      • Fc gamma RIIa antibody
      • Fc gamma RIIb antibody
      • Fc gamma RIII beta antibody
      • Fc-gamma RII-a antibody
      • Fc-gamma-RIIa antibody
      • FCG2A_HUMAN antibody
      • FcGR antibody
      • FCGR2A antibody
      • FCGR2B antibody
      • FCGR3 antibody
      • FCGR3A antibody
      • FCGR3B antibody
      • FcRII-a antibody
      • FcRIII antibody
      • IgG Fc receptor II-a antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-a antibody
      see all

    图片

    • Western blot - Anti-CD16+CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
      Western blot - Anti-CD16+CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
      All lanes : Anti-CD16+CD32 antibody [EPR23501-203] (ab223200) at 1/1000 dilution

      Lane 1 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate
      Lane 2 : bEnd.3 (mouse brain endothelioma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Observed band size: 40-60 kDa why is the actual band size different from the predicted?



      This data was developed using ab223200, the same antibody clone in a different buffer formulation.

      Blocking/Diluting buffer and concentration 5% NFDM/TBST

      The molecular weight observed is consistent with what has been described in the literature (PMID: 19503602, 31172847). Negative control: bEnd.3 (PMID: 23911392).

      Exposure time: Lanes 1-2: 5.5 seconds

    • Flow Cytometry - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
      Flow Cytometry - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)

      This data was developed using ab223200, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of mouse splenocytes cells labelling CD16+CD32 with ab223200 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

      Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

    • Flow Cytometry - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
      Flow Cytometry - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)

      This data was developed using ab223200, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of bEnd.3 (mouse brain endothelioma, Left) / J774A.1 (mouse reticulum cell sarcoma monocyte macrophage, Right) cells labelling CD16+CD32 with ab223200 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730)  isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      Negative control: bEnd.3 (PMID: 23911392).

      Gated on viable cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)

      This data was developed using ab223200, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD16+CD32 with ab223200 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic and membranouse staining on mouse spleen (PMID: 22618994). The section was incubated with ab223200 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD16+CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
      Immunocytochemistry/ Immunofluorescence - Anti-CD16+CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)

      This data was developed using ab223200, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) cells labelling CD16+CD32 with ab223200 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong membranous and weak cytoplasmic staining in J774A.1 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000  dilution.

      Negative control: bEnd.3 cell (PMID: 23911392).

    • Immunoprecipitation - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
      Immunoprecipitation - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)

      This data was developed using ab223200, the same antibody clone in a different buffer formulation.

      CD16+CD32 was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab223200 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab223200 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse spleen tissue lysate 10 ug

      Lane 2: ab223200 IP in Mouse spleen tissue lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223200 in mouse spleen tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes.

       

    • Western blot - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
      Western blot - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
      All lanes : Anti-CD16+CD32 antibody [EPR23501-203] (ab223200) at 1/1000 dilution

      Lane 1 : His-tagged mouse CD16 recombinant protein (aa 31-215), 5 uL
      Lane 2 : His-tagged mouse CD32 recombinant protein (aa 30-210), 25 uL

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution


      This data was developed using ab223200, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      This product can recognize both CD16 and CD32.

      The loading samples are E.coil extracts containing CD16 or CD32 recombinant protein respectively.

      Exposure time: 70 seconds.

    • Immunoprecipitation - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
      Immunoprecipitation - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)

      This data was developed using ab223200, the same antibody clone in a different buffer formulation.

      CD16+CD32 was immunoprecipitated from 0.35 mg J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate with ab223200 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab223200 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate 10 ug

      Lane 2: ab223200 IP in J774A.1 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223200 in J774A.1 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 8 seconds.

       

    • Western blot - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
      Western blot - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
      All lanes : Anti-CD16+CD32 antibody [EPR23501-203] (ab223200) at 1/1000 dilution

      Lane 1 : Mouse spleen tissue lysate
      Lane 2 : Mouse liver tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

      Observed band size: 40-60 kDa why is the actual band size different from the predicted?



      This data was developed using ab223200, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 19503602, 31172847).

      Exposure time: 3 minutes.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)

      This data was developed using ab223200, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse colon carcinoma tissue labeling CD16+CD32 with ab223200 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the stroma in mouse colon carcinoma. The section was incubated with ab223200 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)

      This data was developed using ab223200, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD16+CD32 with ab223200 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse liver. The section was incubated with ab223200 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-CD16+CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)
      Anti-CD16+CD32 antibody [EPR23501-203] - BSA and Azide free (ab275095)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab275095？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab275095 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    There are currently no Customer reviews or Questions for ab275095.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com