重组Anti-CD168抗体[EPR4055] - BSA and Azide free (ab271875)
![Western blot - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)](https://www.abcam.cn/ps/products/271/ab271875/Images/ab271875-483099-anticd168antibodyepr4055westernblot.jpg "Western blot - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4055] to CD168 - BSA and Azide free
- Suitable for: WB, IP, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD168抗体[EPR4055] - BSA and Azide free
参阅全部 CD168 一抗 -
描述
兔单克隆抗体[EPR4055] to CD168 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- T47-D MCF-7, SKBR-3 and LnCaP cell lysate Paraffin-embedded human breast carcinoma tissue Paraffin-embedded human testis tissue WB: Wild type HAP1 whole cell lysate.
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常规说明
ab271875 is the carrier-free version of ab108339.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4055 -
同种型
IgG -
研究领域
相关产品
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Affibody® Molecule
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab271875于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.
|
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| IP |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 84 kDa. |
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IP
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including the focal adhesion kinase occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity. -
组织特异性
Expressed in breast cancer cell lines and in normal breast tissue. -
细胞定位
Cell surface. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 3161 Human
- Omim: 600936 Human
- SwissProt: O75330 Human
- Unigene: 728200 Human
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别名
- CD168 antibody
- CD168 antigen antibody
- HMMR antibody
see all
图片
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Western blot - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108339).
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Empty knockout HAP1 whole cell lysate (20 µg)
Lane 3: CD168 whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab108339 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab108339 was shown to specifically react with CD168 when CD168 knockout samples were used. Wild-type and Empty knockout samples were subjected to SDS-PAGE. Ab108339 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)](https://www.abcam.cn/ps/products/271/ab271875/Images/ab271875-1-ab108339120934anticd168antibodyepr4055immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)ab108339, at 1/100, staining CD168 in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108339). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)](https://www.abcam.cn/ps/products/271/ab271875/Images/ab271875-2-ab108339120933anticd168antibodyepr4055immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)ab108339, at 1/100, staining CD168 in paraffin-embedded human testis tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108339). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)](https://www.abcam.cn/ps/products/271/ab271875/Images/ab271875-3-ab108339120932anticd168antibodyepr4055immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)ab108339 showing positive staining in Normal thymus tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108339).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)](https://www.abcam.cn/ps/products/271/ab271875/Images/ab271875-4-ab108339120931anticd168antibodyepr4055immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)ab108339 showing positive staining in Normal stomach tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108339).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)](https://www.abcam.cn/ps/products/271/ab271875/Images/ab271875-5-ab108339120930anticd168antibodyepr4055immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)ab108339 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108339).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)](https://www.abcam.cn/ps/products/271/ab271875/Images/ab271875-6-ab108339120929anticd168antibodyepr4055immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)ab108339 showing positive staining in Colonic adenocarcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108339).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)](https://www.abcam.cn/ps/products/271/ab271875/Images/ab271875-7-ab108339120928anticd168antibodyepr4055immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)ab108339 showing negative staining in Normal liver tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108339).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)](https://www.abcam.cn/ps/products/271/ab271875/Images/ab271875-8-ab108339120927anticd168antibodyepr4055immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)ab108339 showing positive staining in Normal tonsil tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108339).
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![Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)](https://www.abcam.cn/ps/products/271/ab271875/Images/ab271875-9-benefits-of-recombinant-antibodies.png)
Anti-CD168 antibody [EPR4055] - BSA and Azide free (ab271875)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab271875 尚未被引用在任何文献中。
