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使用敲除细胞株进行验证重组RabMAb

重组Anti-CD168抗体[EPR4054] - BSA and Azide free (ab271887)

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Western blot - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
  • Western blot - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
  • Western blot - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
  • Immunoprecipitation - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
  • OI-RD Scanning - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
  • Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4054] to CD168 - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Unconjugated

概述

  • 产品名称

    Anti-CD168抗体[EPR4054] - BSA and Azide free
    参阅全部 CD168 一抗

  • 描述

    兔单克隆抗体[EPR4054] to CD168 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, IP, WBmore details
    不适用于: Flow Cyt or ICC/IF

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IP: MCF-7. IHC-P: Human gastric carcinoma tissue. Human testis tissue.

  • 常规说明

    ab271887 is the carrier-free version of ab124729.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab271887于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration.

Antigen retrieval is recommended

 
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.

The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

 
说明
IHC-P
Use at an assay dependent concentration.

Antigen retrieval is recommended

 
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.

The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

 
应用说明
Is unsuitable for Flow Cyt or ICC/IF.

靶标

  • 功能

    Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including the focal adhesion kinase occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity.

  • 组织特异性

    Expressed in breast cancer cell lines and in normal breast tissue.

  • 细胞定位

    Cell surface. Cytoplasm.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • CD168 antibody
    • CD168 antigen antibody
    • HMMR antibody
    • HMMR_HUMAN antibody
    • Hyaluronan mediated motility receptor antibody
    • Hyaluronan-mediated motility receptor (RHAMM) antibody
    • IHABP antibody
    • Intracellular hyaluronic acid-binding protein antibody
    • MGC119494 antibody
    • MGC119495 antibody
    • OTTHUMP00000196920 antibody
    • Receptor for hyaluronan-mediated motility antibody
    • RHAMM antibody
    see all

图片

  • Western blot - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
    Western blot - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
    All lanes : Anti-CD168 antibody [EPR4054] (ab124729) at 1/1000 dilution

    Lane 1 : MDA-MB-435 (Human mammary gland ductal carcinoma melanocyte) whole cell lysates at 20 µg
    Lane 2 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 15 µg
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysates at 15 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 84 kDa



    This data was developed using ab124729, the same antibody clone in a different buffer formulation:

    5% NFDM/TBST

  • Western blot - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
    Western blot - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
    All lanes : Anti-CD168 antibody [EPR4054] (ab124729)

    Lane 1 : MDA-MB-435 cell lysate
    Lane 2 : LnCaP cell lysate
    Lane 3 : Sample: T47-D cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 84 kDa

  • Western blot - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
    Western blot - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)

    This data was developed using ab124729, the same antibody clone in a different buffer formulation:

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Empty
    Lane 3: CD168 knockout HAP1 whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab124729 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab124729 was shown to specifically react with CD168 when CD168 knockout samples were used. Wild-type and CD168 knockout samples were subjected to SDS-PAGE. Ab124729 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
    Immunoprecipitation - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)

    ab124729 (purified) at 1:100 dilution (2ug) immunoprecipitating CD168 in MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate.

    Lane 1 (input): MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10ug

    Lane 2 (+): ab124729 & MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124729 in MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124729).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric carcinoma tissue sections labeling CD168 with purified  ab124729 at 1:250 dilution (7.7 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124729).
  • OI-RD Scanning - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
    OI-RD Scanning - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124729).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)

    Immunohistochemical analysis of paraffin-embedded human testis tissue using unpurified ab124729 at 1/50 - 1/100 dilution

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124729).

  • Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
    Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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