Anti-CD16抗体[3G8] - Low endotoxin，Azide free (ab176528)
Key features and details
- Mouse monoclonal [3G8] to CD16 - Low endotoxin, Azide free
- Suitable for: IP, Functional Studies, IHC-Fr, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-CD16抗体[3G8] - Low endotoxin，Azide free
参阅全部 CD16 一抗 -
描述
小鼠单克隆抗体[3G8] to CD16 - Low endotoxin，Azide free -
宿主
Mouse -
经测试应用
适用于: IP, Functional Studies, IHC-Fr, Flow Cytmore details -
种属反应性
与反应: Human
预测可用于: Non human primates
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免疫原
Tissue, cells or virus corresponding to Human CD16. Human neutrophils.
Database link: P08637 -
常规说明
Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
ab176528 is purified from hybridoma culture supernatant by protein-A affinity chromatography. Purity: > 95% (by SDS-PAGE). -
克隆
单克隆 -
克隆编号
3G8 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
- PE Anti-CD16 antibody [3G8] (ab117117)
- PerCP Anti-CD16 antibody [3G8] (ab130761)
- PerCP/Cy5.5® Anti-CD16 antibody [3G8], prediluted (ab157322)
- Alexa Fluor® 488 Anti-CD16 antibody [3G8] (ab187585)
- APC/Cy7® Anti-CD16 antibody [3G8], prediluted (ab197141)
- PE/Cy7® Anti-CD16 antibody [3G8] (ab234209)
- FITC Anti-CD16 antibody [3G8] (ab239254)
- Alexa Fluor® 647 Anti-CD16 antibody [3G8] (ab239275)
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176528于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
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| Functional Studies |
Use at an assay dependent concentration.
In vitro Stimulation of NK cell proliferation, blocking of IgG binding and phagocytosis, inhibition of cytotoxic activity, in vivo NK cell depletion. |
|
| IHC-Fr |
Use at an assay dependent concentration.
Acetone fixation. |
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
IP
Use at an assay dependent concentration. |
|
Functional Studies
Use at an assay dependent concentration. In vitro Stimulation of NK cell proliferation, blocking of IgG binding and phagocytosis, inhibition of cytotoxic activity, in vivo NK cell depletion. |
|
IHC-Fr
Use at an assay dependent concentration. Acetone fixation. |
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. -
组织特异性
Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts. -
序列相似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
翻译后修饰
Glycosylated. Contains high mannose- and complex-type oligosaccharides.
The soluble form is produced by a proteolytic cleavage. -
细胞定位
Cell membrane. Secreted. Exists also as a soluble receptor. - Information by UniProt
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数据库链接
- Entrez Gene: 2214 Human
- Omim: 146740 Human
- SwissProt: P08637 Human
- Unigene: 372679 Human
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别名
- CD 16 antibody
- CD 16a antibody
- CD16 antibody
see all
实验方案
数据表及文件
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Datasheet download
文献 (0)
ab176528 尚未被引用在任何文献中。
