Anti-CD15抗体[P12] (ab3358)
Key features and details
- Mouse monoclonal [P12] to CD15
- Suitable for: IHC-P, ICC/IF
- Isotype: IgM
概述
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产品名称
Anti-CD15抗体[P12]
参阅全部 CD15 一抗 -
描述
小鼠单克隆抗体[P12] to CD15 -
宿主
Mouse -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
预测可用于: Human -
免疫原
Fresh (non-cultured) placenta cell preparation (Human).
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阳性对照
- Human kidney tissue.
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常规说明
Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. This antibody belongs to the BG-7 group and detects the Lewis x (type 2 chain) blood-group antigen, which is expressed in gastrointestinal tumors, and lung cancers. It is found in crypt cells, which represent the proliferative compartment of the colonic mucosa, and strong homogeneous immunostaining is observed in most metastases and invasive cancer cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Tissue culture supernatant -
Primary antibody说明
Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. This antibody belongs to the BG-7 group and detects the Lewis x (type 2 chain) blood-group antigen, which is expressed in gastrointestinal tumors, and lung cancers. It is found in crypt cells, which represent the proliferative compartment of the colonic mucosa, and strong homogeneous immunostaining is observed in most metastases and invasive cancer cells. -
克隆
单克隆 -
克隆编号
P12 -
同种型
IgM -
研究领域
相关产品
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Compatible Secondaries
应用
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/40.
|
|
ICC/IF |
1/200 - 1/400.
|
说明 |
---|
IHC-P
1/40. |
ICC/IF
1/200 - 1/400. |
靶标
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相关性
CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes. -
细胞定位
Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi. -
别名
- 3 fucosyl N acetyl lactosamine antibody
- 3-FAL antibody
- 3-fucosyl-N-acetyl-lactosamine antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab3358 被引用在 5 文献中.
- Lu HH et al. Fucosyltransferase 4 shapes oncogenic glycoproteome to drive metastasis of lung adenocarcinoma. EBioMedicine 57:102846 (2020). PubMed: 32629386
- Purohit S et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). PubMed: 29343722
- Morelle W et al. Glycosylation pattern of brush border-associated glycoproteins in enterocyte-like cells: involvement of complex-type N-glycans in apical trafficking. Biol Chem 390:529-44 (2009). PubMed: 19426135
- Shi CS et al. Lectin-like domain of thrombomodulin binds to its specific ligand Lewis Y antigen and neutralizes lipopolysaccharide-induced inflammatory response. Blood 112:3661-70 (2008). PubMed: 18711002
- Nyström K et al. Virus-induced transcriptional activation of host FUT genes associated with neo-expression of Ley in cytomegalovirus-infected and sialyl-Lex in varicella-zoster virus-infected diploid human cells. Glycobiology 17:355-66 (2007). PubMed: 17202152