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Tags & Cell Markers Cell Type Markers Tumor Associated

Anti-CD15抗体[MEM-158] (ab665)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

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Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)
  • Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)

Key features and details

  • Mouse monoclonal [MEM-158] to CD15
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgM

Conjugates logo Related conjugates and formulations

APC Biotin FITC PerCP/Cy5.5®

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概述

  • 产品名称

    Anti-CD15抗体[MEM-158]
    参阅全部 CD15 一抗
  • 描述

    小鼠单克隆抗体[MEM-158] to CD15
  • 宿主

    Mouse
  • 经测试应用

    适用于: Flow Cytmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Tissue, cells or virus corresponding to Human CD15. Human granulocytes

  • 阳性对照

    • Flow Cyt: Human peripheral whole blood.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 8.00
    Preservative: 0.097% Sodium azide
    Constituent: Tris buffered saline
  • Concentration information loading...
  • 纯度

    Proprietary Purification
  • 纯化说明

    Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE.
  • 克隆

    单克隆
  • 克隆编号

    MEM-158
  • 同种型

    IgM
  • 研究领域

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Cancer
    • Tumor immunology
    • CD markers
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers

相关产品

  • Alternative Versions

    • FITC Anti-CD15 antibody [MEM-158] (ab18272)
    • PerCP/Cy5.5® Anti-CD15 antibody [MEM-158] (ab234266)
    • Biotin Anti-CD15 antibody [MEM-158] (ab28103)
    • APC Anti-CD15 antibody [MEM-158] (ab88032)
  • Compatible Secondaries

    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    • Goat Anti-Mouse IgM H&L (ab9167)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab665于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use a concentration of 1 - 4 µg/ml.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

说明
Flow Cyt
Use a concentration of 1 - 4 µg/ml.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性

    CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
  • 细胞定位

    Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
  • 别名

    • 3 fucosyl N acetyl lactosamine antibody
    • 3-FAL antibody
    • 3-fucosyl-N-acetyl-lactosamine antibody
    • Leu M1 antibody
    • Lewis X antibody
    • LeX antibody
    • SSEA-1 antibody
    • stage-specific embryonic antigen 1 antibody
    see all

图片

  • Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)
    Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)

    Flow Cytometry analysis of human peripheral whole blood labeling CD15 (surface staining) with ab665 at 0.3 µg/mL. CD15 positive human neutrophil granulocytes (red-filled). CD15 negative lymphocytes (black-dashed).

  • Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)
    Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)
    Flow cytometry surface staining pattern of human peripheral blood stained using Anti-CD15 antibody [MEM-158] (ab665) at 0.3 µg/mL.

实验方案

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (1)

发表研究结果有使用 ab665?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab665 被引用在 1 文献中.

  • Kishimoto T. (Eds.) et al. Leucocyte typing VI. Garland Publishing Inc. : (1997). PubMed:

客户评价及客户问答

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Immunocytochemistry/ Immunofluorescence abreview for Anti-CD15 antibody [MEM-158]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Cord blood)
Specification
Cord blood
Fixative
Methanol
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 May 25 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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