Anti-CD15抗体[BRA-4F1] (ab115993)
Key features and details
- Mouse monoclonal [BRA-4F1] to CD15
- Suitable for: WB, IHC-P, IHC-Fr, ICC, ICC/IF
- Reacts with: Human
- Isotype: IgM
概述
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产品名称
Anti-CD15抗体[BRA-4F1]
参阅全部 CD15 一抗 -
描述
小鼠单克隆抗体[BRA-4F1] to CD15 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, IHC-Fr, ICC, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD15. Erythroid myeloid leukemia cells line K562.
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阳性对照
- Reed Sternberg cells in Hodgkin’s lymphoma tissue.
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常规说明
This product was changed from ascites to tissue culture supernatant on 15th June 2017. Lot numbers higher than GR177792-4 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
BRA-4F1 -
同种型
IgM -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115993于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/100 - 1/200. Predicted molecular weight: 59 kDa.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| IHC-Fr |
1/50 - 1/100.
|
|
| ICC |
Use at an assay dependent concentration.
(methanol fixed) Use at an assay dependent concentration |
|
| ICC/IF |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
1/100 - 1/200. Predicted molecular weight: 59 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IHC-Fr
1/50 - 1/100. |
|
ICC
Use at an assay dependent concentration. (methanol fixed) Use at an assay dependent concentration |
|
ICC/IF
Use at an assay dependent concentration. |
靶标
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相关性
CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes. -
细胞定位
Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi. -
别名
- 3 fucosyl N acetyl lactosamine antibody
- 3-FAL antibody
- 3-fucosyl-N-acetyl-lactosamine antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab115993 尚未被引用在任何文献中。