Anti-CD147抗体[MEM-M6/6] - Low endotoxin，Azide free (ab119114)
![Flow Cytometry - Anti-CD147 antibody [MEM-M6/6] - Low endotoxin, Azide free (ab119114)](https://www.abcam.cn/ps/products/119/ab119114/Images/ab119114-199432-anti-cd147-antibody-mem-m6-6-flow-cytometry.jpg "Flow Cytometry - Anti-CD147 antibody [MEM-M6/6] - Low endotoxin, Azide free (ab119114)")
Key features and details
- Mouse monoclonal [MEM-M6/6] to CD147 - Low endotoxin, Azide free
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human, Simian Virus 40
- Isotype: IgG1
概述
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产品名称
Anti-CD147抗体[MEM-M6/6] - Low endotoxin，Azide free
参阅全部 CD147 一抗 -
描述
小鼠单克隆抗体[MEM-M6/6] to CD147 - Low endotoxin，Azide free -
宿主
Mouse -
特异性
ab119114 recognizes Ig domain D2 (membrane proximal) of CD147 (Neurothelin).
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经测试应用
适用于: Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Human, Simian Virus 40 -
免疫原
Protein A-CR purified soluble recombinant Human CD147 (consisting of the CDNA encoding the hinge region, CH2-and CH3 domain of Human IgG1).
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阳性对照
- Flow Cyt: Human peripheral blood lymphocytes ICC/IF: SV40 cells.
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常规说明
Endotoxin level is less than 10 EU/mg of the protein, as determined by the LAL test.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 99% PBS
Azide-free PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
ab119114 is Purified from hybridoma culture supernatant by protein-A affinity chromatography. Purity is > 95% (by SDS-PAGE), 0.2 µm filter sterilized. -
克隆
单克隆 -
克隆编号
MEM-M6/6 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119114于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| 说明 |
|---|
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. -
组织特异性
Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas. -
序列相似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
N-glycosylated. -
细胞定位
Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 682 Human
- Omim: 109480 Human
- SwissProt: P35613 Human
- Unigene: 501293 Human
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别名
- 5A11 antigen antibody
- 5F7 antibody
- BASI_HUMAN antibody
see all
图片
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Flow Cytometry - Anti-CD147 antibody [MEM-M6/6] - Low endotoxin, Azide free (ab119114)
Human peripheral blood lymphocytes stained with ab119114 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab119114, 1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [MEM-M6/6] - Low endotoxin, Azide free (ab119114)](https://www.abcam.cn/ps/products/119/ab119114/Images/ab119114-132024-anti-cd147-antibody-mem-m6-6-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [MEM-M6/6] - Low endotoxin, Azide free (ab119114)ICC/IF image of ab119114 stained SV40 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab119114, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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Datasheet download
文献 (8)
ab119114 被引用在 8 文献中.
- Shilts J et al. No evidence for basigin/CD147 as a direct SARS-CoV-2 spike binding receptor. Sci Rep 11:413 (2021). PubMed: 33432067
- Moore AJ et al. Assessing the functional impact of PfRh5 genetic diversity on ex vivo erythrocyte invasion inhibition. Sci Rep 11:2225 (2021). PubMed: 33500482
- Chen H et al. CD147 deficiency is associated with impairedsperm motility/acrosome reaction and offersa therapeutic target for asthenozoospermia. Mol Ther Nucleic Acids 26:1374-1386 (2021). PubMed: 34900396
- Teymournejad O & Rikihisa Y Ehrlichia chaffeensis Uses an Invasin To Suppress Reactive Oxygen Species Generation by Macrophages via CD147-Dependent Inhibition of Vav1 To Block Rac1 Activation. mBio 11:N/A (2020). PubMed: 32317318
- Pahk K et al. SP-8356, a Novel Inhibitor of CD147-Cyclophilin A Interactions, Reduces Plaque Progression and Stabilizes Vulnerable Plaques in apoE-Deficient Mice. Int J Mol Sci 21:N/A (2019). PubMed: 31877775
- Awandare GA et al. Plasmodium falciparum strains spontaneously switch invasion phenotype in suspension culture. Sci Rep 8:5782 (2018). PubMed: 29636510
- Sharma S et al. Genome-scale identification of cellular pathways required for cell surface recognition. Genome Res 28:1372-1382 (2018). PubMed: 29914970
- Updegraff BL et al. Transmembrane Protease TMPRSS11B Promotes Lung Cancer Growth by Enhancing Lactate Export and Glycolytic Metabolism. Cell Rep 25:2223-2233.e6 (2018). PubMed: 30463017
