Anti-CD147抗体[MEM-M6/2] (ab21903)
![Flow Cytometry - Anti-CD147 antibody [MEM-M6/2] (ab21903)](https://www.abcam.cn/ps/products/21/ab21903/Images/ab21903-2-Anti-CD147-antibody-MEM-M62-Human-A549-cells-flow-cytometry.jpg "Flow Cytometry - Anti-CD147 antibody [MEM-M6/2] (ab21903)")
Key features and details
- Mouse monoclonal [MEM-M6/2] to CD147
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-CD147抗体[MEM-M6/2]
参阅全部 CD147 一抗 -
描述
小鼠单克隆抗体[MEM-M6/2] to CD147 -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
A soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the entire extracellular region of CD147 fused to the DNA coding for the hinge region, CH2 and CH3 domain of human IgG1.
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表位
This antibody recognises extracellular Ig domain D1 of CD147 antigen. -
阳性对照
- Flow Cyt: A549 cells.
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常规说明
This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
> 95% (by SDS-PAGE). Purified from TCS -
克隆
单克隆 -
克隆编号
MEM-M6/2 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab21903于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use a concentration of 5 µg/ml.
|
| 说明 |
|---|
|
Flow Cyt
Use a concentration of 5 µg/ml. |
靶标
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功能
Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. -
组织特异性
Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas. -
序列相似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
N-glycosylated. -
细胞定位
Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 682 Human
- Omim: 109480 Human
- SwissProt: P35613 Human
- Unigene: 501293 Human
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别名
- 5A11 antigen antibody
- 5F7 antibody
- BASI_HUMAN antibody
see all
图片
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Flow Cytometry - Anti-CD147 antibody [MEM-M6/2] (ab21903)
Flow cytometry overlay histogram showing wild-type A549 (green line) and BSG knockout A549 cells (ab273748) stained with ab21903 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab21903) (1x106 in 100μl at 5 μg/ml) for 30 min at 4°C.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min at 4°C.
Isotype control antibody was mouse IgG2bκ (ab170192) used at the same concentration and conditions as the primary antibody (wild-type A549 - black line; BSG knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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