Anti-CD147抗体[MEM-M6/1] (ab666)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [MEM-M6/1] (ab666)](https://www.abcam.cn/ps/products/0/ab666/Images/ab666-3597-anti-cd147-antibody-mem-m6-1-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [MEM-M6/1] (ab666)")
Key features and details
- Mouse monoclonal [MEM-M6/1] to CD147
- Suitable for: IHC-P, Flow Cyt, WB, IP
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-CD147抗体[MEM-M6/1]
参阅全部 CD147 一抗 -
描述
小鼠单克隆抗体[MEM-M6/1] to CD147 -
宿主
Mouse -
特异性
Human CD147 antigen. This antibody recognizes an epitope in the N-terminal Ig domain (D1). This high-affinity antibody is capable of binding to unstimulated peripheral blood T cells. -
经测试应用
适用于: IHC-P, Flow Cyt, WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human CD147. Purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimer).
Database link: P35613 -
阳性对照
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle. Flow Cytometry: A549 cells and Peripheral blood lymphocytes. WB: A549, Raji and Jurkat.
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常规说明
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from TCS. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
MEM-M6/1 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (3) |
Use a concentration of 10 µg/ml.
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| Flow Cyt |
Use a concentration of 10 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| WB | (1) |
Use a concentration of 1 µg/ml. Use under non reducing condition.
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| IP |
Use at an assay dependent concentration.
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| 说明 |
|---|
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IHC-P
Use a concentration of 10 µg/ml. |
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Flow Cyt
Use a concentration of 10 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB
Use a concentration of 1 µg/ml. Use under non reducing condition. |
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IP
Use at an assay dependent concentration. |
靶标
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功能
Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. -
组织特异性
Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas. -
序列相似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
N-glycosylated. -
细胞定位
Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 682 Human
- Omim: 109480 Human
- SwissProt: P35613 Human
- Unigene: 501293 Human
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别名
- 5A11 antigen antibody
- 5F7 antibody
- BASI_HUMAN antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [MEM-M6/1] (ab666)
IHC image of CD147 staining in human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab666, 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Western blot - Anti-CD147 antibody [MEM-M6/1] (ab666)](https://www.abcam.cn/ps/products/0/ab666/Images/ab666-446560-anti-cd147-antibody-mem-m61-western-blot-mps-a549-wt-mps-a549-cd147bsg-knockout-raji-jurkat.jpg)
Western blot - Anti-CD147 antibody [MEM-M6/1] (ab666)All lanes : Anti-CD147 antibody [MEM-M6/1] (ab666) at 1 µg/ml
Lane 1 : Wild-type A549 cell lysate
Lane 2 : BSG knockout A549 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Observed band size: 55-70 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab666 observed at 55-70 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab666 was shown to react with CD147 in wild-type A549 cells in western blot with loss of signal observed in BSG knockout cell line ab273748 (knockout cell lysate ab275500). Wild-type and BSG knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab666 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry - Anti-CD147 antibody [MEM-M6/1] (ab666)](https://www.abcam.cn/ps/products/0/ab666/Images/ab666-4-Anti-CD147-antibody-MEM-M61-Human-A549-cells-flow-cytometry.jpg)
Flow Cytometry - Anti-CD147 antibody [MEM-M6/1] (ab666)Flow cytometry overlay histogram showing wild-type A549 (green line) and BSG knockout A549 cells (ab273748) stained with ab666 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab666) (1x106 in 100μl at 10 μg/ml) for 30 min at 4°C.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min at 4°C.
Isotype control antibody was mouse IgG1κ (ab170190) used at the same concentration and conditions as the primary antibody (wild-type A549 - black line; BSG knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
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![Flow Cytometry - Anti-CD147 antibody [MEM-M6/1] (ab666)](https://www.abcam.cn/ps/products/0/ab666/Images/ab666-331842-anti-cd147-antibody-mem-m6-1-flow-cytometry.jpg)
Flow Cytometry - Anti-CD147 antibody [MEM-M6/1] (ab666)Flow cytometry of human peripheral blood cells with ab666 at 1 µg/ml -
![Flow Cytometry - Anti-CD147 antibody [MEM-M6/1] (ab666)](https://www.abcam.cn/ps/products/0/ab666/Images/ab666-3598-anti-cd147-antibody-mem-m6-1-flow-cytometry.jpg)
Flow Cytometry - Anti-CD147 antibody [MEM-M6/1] (ab666)Overlay histogram showing peripheral blood lymphocytes stained with ab666 (red line). The cells were incubated with the antibody (ab666, 1 µg/1x106 cells) for 30 minutes at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab666 被引用在 28 文献中.
- Lu L et al. Novel Functions of CD147 in the Mitochondria Exacerbates Melanoma Metastasis. Int J Biol Sci 17:285-297 (2021). PubMed: 33390850
- Shilts J et al. No evidence for basigin/CD147 as a direct SARS-CoV-2 spike binding receptor. Sci Rep 11:413 (2021). PubMed: 33432067
- Dobrindt K et al. Common genetic variation in humans impacts in vitro susceptibility to SARS-CoV-2 infection. bioRxiv N/A:N/A (2020). PubMed: 32995783
- Colangelo NW & Azzam EI Extracellular vesicles originating from glioblastoma cells increase metalloproteinase release by astrocytes: the role of CD147 (EMMPRIN) and ionizing radiation. Cell Commun Signal 18:21 (2020). PubMed: 32033611
- Aguiar JA et al. Gene expression and in situ protein profiling of candidate SARS-CoV-2 receptors in human airway epithelial cells and lung tissue. Eur Respir J 56:N/A (2020). PubMed: 32675206