Anti-CD14抗体[UCHM1] (ab6623)

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Flow Cytometry - Anti-CD14 antibody [UCHM1] (ab6623)

    Key features and details

    • Mouse monoclonal [UCHM1] to CD14
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG2a

    概述

    • 产品名称

      Anti-CD14抗体[UCHM1]
      参阅全部 CD14 一抗

    • 描述

      小鼠单克隆抗体[UCHM1] to CD14

    • 宿主

      Mouse

    • 特异性

      ab6623 recognises a monocyte surface antigen of 55 kDa found chiefly on monocytes. UCHM1 was clustered at the Third International Workshop and Conference on Human Leucocyte Differentiation Antigens. Oxford 1986, as CDw14, group A. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative. UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver.

    • 经测试应用

      适用于: Flow Cytmore details
      不适用于: IHC-P

    • 种属反应性

      与反应: Human

    • 免疫原

      Tissue, cells or virus corresponding to Human CD14. Human Thymocytes followed by peripheral blood mononuclear cells

    • 阳性对照

      • Tonsil.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6623于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    Use at an assay dependent concentration.

    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
    说明
    Flow Cyt
    Use at an assay dependent concentration.

    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
    应用说明
    Is unsuitable for IHC-P.

    靶标

    • 功能

      Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

    • 组织特异性

      Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

    • 序列相似性

      Contains 11 LRR (leucine-rich) repeats.

    • 翻译后修饰

      N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

    • 细胞定位

      Cell membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • CD 14 antibody
        • CD_antigen=CD14 antibody
        • CD14 antibody
        • CD14 antigen antibody
        • CD14 molecule antibody
        • CD14_HUMAN antibody
        • LPS-R antibody
        • Mo2 antibody
        • Monocyte differentiation antigen CD14 antibody
        • Monocyte differentiation antigen CD14 urinary form antibody
        • Monocyte differentiation antigen CD14, membrane-bound form antibody
        • Myeloid cell specific leucine rich glycoprotein antibody
        • Myeloid cell-specific leucine-rich glycoprotein antibody
        see all

      图片

      • Flow Cytometry - Anti-CD14 antibody [UCHM1] (ab6623)
        Flow Cytometry - Anti-CD14 antibody [UCHM1] (ab6623)
        ab6623 staining of human peripheral blood monocytes, visualised with rabbit anti-mouse IgG: FITC.

      文献 (0)

      发表研究结果有使用 ab6623？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab6623 尚未被引用在任何文献中。

      客户评价及客户问答

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      Question

      Can this antibody be used with paraffin sections or only frozen as stated on the datasheet?

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      Answer

      This antibody has been tested on paraffin sections and does not work. In IHC, it is known to work only with frozen sections.

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