Name: Anti-CD14 antibody [EPR3653]
Brand: RabMAb
SKU: ab133335
Rating: 4 (1 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3653] to CD14
Suitable for: WB, IHC-P
Reacts with: Human

Alexa Fluor® 594 Carrier Free HRP

产品名称

Anti-CD14抗体[EPR3653]
参阅全部 CD14 一抗
描述

兔单克隆抗体[EPR3653] to CD14
宿主

Rabbit
经测试应用

适用于: WB, IHC-Pmore details
不适用于: ICC/IF
种属反应性

与反应: Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- IHC-P: human colon, placenta and tonsil tissue; WB: Human tonsil tissue lysate, SW480 and PBMCl lysates.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
解离常数（K_D）

K_D = 4.70 x 10 ^-12 M
Learn more about K_D
存储溶液

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR3653
同种型

IgG
研究领域

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Mouse CD14 protein (ab207103)

The Abpromise guarantee

Abpromise™承诺保证使用ab133335于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB		1/1000 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 40 kDa).
IHC-P	(1)	1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/500 - 1/1000.

说明
WB 1/1000 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 40 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/500 - 1/1000.

应用说明

Is unsuitable for ICC/IF.

功能

Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
组织特异性

Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
序列相似性

Contains 11 LRR (leucine-rich) repeats.
翻译后修饰

N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
细胞定位

Cell membrane.
Target information above from: UniProt accession P08571 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 929 Human
- Omim: 158120 Human
- SwissProt: P08571 Human
- Unigene: 163867 Human
别名
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
- CD14 antigen antibody
- CD14 molecule antibody
- CD14_HUMAN antibody
- LPS-R antibody
- Mo2 antibody
- Monocyte differentiation antigen CD14 antibody
- Monocyte differentiation antigen CD14 urinary form antibody
- Monocyte differentiation antigen CD14, membrane-bound form antibody
- Myeloid cell specific leucine rich glycoprotein antibody
- Myeloid cell-specific leucine-rich glycoprotein antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling CD14 with purified ab133335 at 1/2000 dilution (0.04 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-CD14 antibody [EPR3653] (ab133335)

Anti-CD14 antibody [EPR3653] (ab133335) at 1/1000 dilution (purified) + His-Tagged Human CD14 (aa20 to 345) recombinant protein at 0.015 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 40 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking and diluting buffer: 5% NFDM/TBST
Western blot - Anti-CD14 antibody [EPR3653] (ab133335)

All lanes : Anti-CD14 antibody [EPR3653] (ab133335) at 1/1000 dilution

Lane 1 : Human tonsil lysates prepared in RIPA lysis method
Lane 2 : Human tonsil lysates prepared in 1%SDS Hot lysis method

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 40 kDa

Exposure time: 45 seconds

Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Western blot - Anti-CD14 antibody [EPR3653] (ab133335)

All lanes : Anti-CD14 antibody [EPR3653] (ab133335) at 1/1000 dilution (purified)

Lane 1 : Human tonsil tissue lysate prepared in 1% SDS Hot lysis method
Lane 2 : HeLa (Human cervix adenocarcinoma) whole cell lysate
Lane 3 : U-937 (Human histiocytic lymphoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lane 1 : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 40 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and diluting buffer: 5% NFDM/TBST

The expression level in HeLa and U-937 are low (PMID: 9886426 and 15730927)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)

Immunohistochemical analysis of Formalin-fixed, paraffin- embedded human tonsil tissue labelling CD14 with ab133335 (unpurified) at 1/500 dilution. No blocking step performed. Anti-Rabbit HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using EDTA based pH 9.0 buffer.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)

Immunohistochemical analysis of Formalin-fixed, paraffin- embedded Human tonsil tissue labelling CD14 with ab133335 (unpurified) at 1/500 dilution. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)

Immunohistochemical analysis of Formalin-fixed, paraffin- embedded Human placenta tissue labelling CD14 with ab133335 (unpurified) at 1/500 dilution. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Western blot - Anti-CD14 antibody [EPR3653] (ab133335)

All lanes : Anti-CD14 antibody [EPR3653] (ab133335) at 1/1000 dilution (unpurified)

Lane 1 : PBMC cell lysate prepared in 1% SDS Hot lysis method
Lane 2 : Human tonsil tissue lysate prepared in 1%SDS Hot lysis method
Lane 3 : SW480 cell lysate prepared in 1%SDS Hot lysis method

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

Predicted band size: 40 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
OI-RD Scanning - Anti-CD14 antibody [EPR3653] (ab133335)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)

Tissue Microarrays stained for "Anti-CD14 antibody [EPR3653]” using "ab133335" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab133335 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Anti-CD14 antibody [EPR3653] (ab133335)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Protocol Booklet

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab133335？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab133335 被引用在 27 文献中.

Zhang L et al. BEST1 Positive Monocytes in Circulation: Visualize Intratumoral Crosstalk between Cancer Cells and Monocytes. Adv Sci (Weinh) 10:e2205915 (2023). PubMed: 37088729
Zwart ES et al. The immune microenvironment after neoadjuvant therapy compared to upfront surgery in patients with pancreatic cancer. J Cancer Res Clin Oncol 149:14731-14743 (2023). PubMed: 37587309
Zwart ES et al. Spatial immune composition of tumor microenvironment in patients with pancreatic cancer. Cancer Immunol Immunother 72:4385-4397 (2023). PubMed: 37938368
Jarosch S et al. ChipCytometry for multiplexed detection of protein and mRNA markers on human FFPE tissue samples. STAR Protoc 3:101374 (2022). PubMed: 35586313
Lopez DC et al. Multi-spectral immunofluorescence evaluation of the myeloid, T cell, and natural killer cell tumor immune microenvironment in chordoma may guide immunotherapeutic strategies. Front Oncol 12:1012058 (2022). PubMed: 36338744

View all Publications for this product

客户评价及客户问答

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Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer ph 8,5

Sample

Human Tissue sections (spleen)

Specification

spleen

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Rudolf Jung

Verified customer

提交于 Oct 22 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

解离常数（KD）

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

组织特异性

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (27)

客户评价及客户问答

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD14 antibody [EPR3653]

解离常数（K_D）