Anti-CD14抗体(ab45870)
Key features and details
- Goat polyclonal to CD14
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-CD14抗体
参阅全部 CD14 一抗 -
描述
山羊多克隆抗体to CD14 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human CD14 aa 50-150 (internal sequence). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
-
阳性对照
- WB: Human lymph node lysate and human tonsil lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45870于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa).
A 1 hour primary incubation is recommended for this product. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun. 2001 Jun;69(6):3772-81; PMID: 11349042). |
说明 |
---|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa). A 1 hour primary incubation is recommended for this product. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun. 2001 Jun;69(6):3772-81; PMID: 11349042). |
靶标
-
功能
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. -
组织特异性
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. -
序列相似性
Contains 11 LRR (leucine-rich) repeats. -
翻译后修饰
N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
细胞定位
Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 929 Human
- Omim: 158120 Human
- SwissProt: P08571 Human
- Unigene: 163867 Human
-
别名
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
see all
图片
-
All lanes : Anti-CD14 antibody (ab45870) at 2 µg/ml
Lane 1 : Human lymph node lysate
Lane 2 : Human lymph node lysate with Immunizing peptide
Lane 3 : Human tonsil lysate
Lane 4 : Human tonsil lysate with Immunizing peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 40 kDa
Additional bands at: 55 kDa (possible glycosylated form)Primary incubation 1 hour at room temperature. Detected by chemiluminescence. RIPA buffer used.
数据表及文件
-
SDS download
-
Datasheet download
文献 (12)
ab45870 被引用在 12 文献中.
- Tanoue S et al. Role of apoptosis inhibitor of macrophages in patients with IgG4‑related disease/autoimmune pancreatitis and the clinical characteristics associated with this condition. Biomed Rep 17:82 (2022). PubMed: 36158321
- Liu M et al. Myeloid-derived suppressor cells in gastroenteropancreatic neuroendocrine neoplasms. Endocrine 71:242-252 (2021). PubMed: 33010002
- Aguirre LA et al. Tumor stem cells fuse with monocytes to form highly invasive tumor-hybrid cells. Oncoimmunology 9:1773204 (2020). PubMed: 32923132
- Hu R et al. Construction of a sensitive pyrogen-testing cell model by site-specific knock-in of multiple genes. Biotechnol Bioeng 116:2652-2661 (2019). PubMed: 31180145
- Han Q et al. Application of a TLR overexpression cell model in pyrogen detection. Biotechnol Bioeng 116:1269-1279 (2019). PubMed: 30684361