重组Anti-CD134 / OX40L receptor抗体[EPR17Y] (ab76000)
![Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)](https://www.abcam.cn/ps/products/76/ab76000/Images/ab76000-330890-anti-cd134-ox40l-receptor-antibody-epr17y-flow-cytometry.jpg "Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17Y] to CD134 / OX40L receptor
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD134 / OX40L receptor抗体[EPR17Y]
参阅全部 CD134 / OX40L receptor 一抗 -
描述
兔单克隆抗体[EPR17Y] to CD134 / OX40L receptor -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt, WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human CD134/ OX40L receptor aa 1-250. The exact sequence is proprietary.
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阳性对照
- WB: Human lymph node, spleen and tonsil tissue lysates and PMBC cell lysate treated with TPA.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.19% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76000于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
|
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| WB |
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 30 kDa).
|
| 说明 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
|
WB
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 30 kDa). |
应用说明
Is unsuitable for ICC/IF or IHC-P.
靶标
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功能
Receptor for TNFSF4/OX40L/GP34. -
序列相似性
Contains 4 TNFR-Cys repeats. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7293 Human
- Omim: 600315 Human
- SwissProt: P43489 Human
- Unigene: 129780 Human
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别名
- ACT 35 antibody
- ACT35 antibody
- ACT35 antigen antibody
see all
图片
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Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)
Flow cytometry analysis of Human PBMC treated with 1©:g/ml PHA for 3 days cells labelling CD134 / OX40L receptor with ab76000 at 1/1200 dilution (1.05 μg/ml) (Red). Dylight488 (Ab98462) was used as the secondary antibody at 1/2000 dilution.
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![Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)](https://www.abcam.cn/ps/products/76/ab76000/Images/ab76000-258722-anti-cd134-ox40l-receptor-antibody-epr17y-western-blot.jpg)
Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)All lanes : Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/5000 dilution
Lane 1 : Human lymph node tissue lysate
Lane 2 : Human spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer 5% NFDM/TBST -
![Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)](https://www.abcam.cn/ps/products/76/ab76000/Images/ab76000-258723-anti-cd134-ox40l-receptor-antibody-epr17y-western-blot.jpg)
Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/1000 dilution + Human tonsil tissue lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer 5% NFDM/TBST -
![Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)](https://www.abcam.cn/ps/products/76/ab76000/Images/ab76000-91259-anti-cd134-ox40l-receptor-antibody-epr17y-western-blot.jpg)
Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)All lanes : Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/500 dilution (unpurified)
Lane 1 : PBMC cell lysates, untreated
Lane 2 : PBMC cell lysates, treated with TPA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Note: the actual molecular weight is larger than the predicted due to glycoslyation. -
![Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)](https://www.abcam.cn/ps/products/76/ab76000/Images/ab76000-5-benefits-of-recombinant-antibodies.png)
Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab76000 被引用在 2 文献中.
- Wang X et al. Effect of cyclosporin A intervention on the immunological mechanisms of coronary heart disease and restenosis. Exp Ther Med 12:3242-3248 (2016). IHC-P ; Rabbit . PubMed: 27882144
- Byun M et al. Inherited human OX40 deficiency underlying classic Kaposi sarcoma of childhood. J Exp Med 210:1743-59 (2013). WB, Flow Cyt ; Human . PubMed: 23897980