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AB317084

Anti-CD133 antibody [HL1271]

Anti-CD133 antibody [HL1271]

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Rabbit Monoclonal CD133 antibody. Suitable for WB and reacts with Transfected cell lysate, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PROM1.

查看别名

CD133, PROML1, MSTP061, PROM1, Prominin-1, Antigen AC133, Prominin-like protein 1

2 Images
Western blot - Anti-CD133 antibody [HL1271] (AB317084)
  • WB

Supplier Data

Western blot - Anti-CD133 antibody [HL1271] (AB317084)

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab317084 diluted at 1 : 1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CD133 antibody [HL1271] (ab317084) at 1/1000 dilution

Lane 1:

NT2D1 whole cell extracts at 30 µg

Lane 2:

293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-CD133 antibody [HL1271] (AB317084)
  • WB

Supplier Data

Western blot - Anti-CD133 antibody [HL1271] (AB317084)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab317085 diluted at 1 : 5000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CD133 antibody [HL1271] (ab317084) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

CD133 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

HL1271

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB

applications

免疫原

Recombinant Fragment Protein within Human PROM1.

O43490

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
存储溶液
pH: 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD133 also known as Prominin-1 is a transmembrane glycoprotein with a molecular weight of approximately 120 kDa. This protein stands out due to its expression in various stem and progenitor cells including those of the neural hematopoietic and epithelial lineages. CD133 is recognized for its distinct five transmembrane domains and two large extracellular loops. Researchers commonly use CD133 as a cell surface marker to identify stem and progenitor cells. The significance of CD133 extends to immunohistochemistry (IHC) and Western blot applications where it is a popular target for detection and analysis.
Biological function summary

CD133 is involved in maintaining the cellular architecture and signaling processes. It plays a role in cellular differentiation and proliferation while acting as a part of larger protein complexes on the cell surface. This protein is notable as it contributes significantly to the regulation of stem cell properties supporting the growth and repair in various tissues. Anti-CD133 antibodies are valuable tools in studying these functions due to their specificity in targeting this marker.

Pathways

CD133 participates in cellular signaling and transport pathways vital for intercellular communication and homeostasis. It interacts within pathways related to cell polarity and asymmetric division both essential for tissue development and maintenance. CD133 is associated with proteins like CD133/1 and CD133/2 which further highlights its involvement in these complex biological networks and on pathways such as the Wnt signaling pathway which regulates gene expression associated with cell proliferation and differentiation.

Several studies indicate a strong relationship between CD133 expression and certain cancers such as glioblastoma and colorectal cancer. In these cases CD133 serves not only as a marker but also as an indicator of the presence of cancer stem cells which are capable of tumor initiation and maintenance. Furthermore researchers have identified connections between CD133 and other proteins such as c-Met (cMab) within these pathological contexts suggesting interactions that could contribute to disease progression and offering potential targets for therapeutic intervention.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

May play a role in cell differentiation, proliferation and apoptosis (PubMed : 24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed : 20818439).
See full target information PROM1

其他靶点

Prom1
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