重组Anti-CD133抗体[EPR20980-45] (ab226355)
![Western blot - Anti-CD133 antibody [EPR20980-45] (ab226355)](https://www.abcam.cn/ps/products/226/ab226355/Images/ab226355-451726-anti-cd133-antibody-epr20980-45-western-blot-wildtype-hap1-prom1-knockout-hap1-caco2-hela.jpg "Western blot - Anti-CD133 antibody [EPR20980-45] (ab226355)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20980-45] to CD133
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD133抗体[EPR20980-45]
参阅全部 CD133 一抗 -
描述
兔单克隆抗体[EPR20980-45] to CD133 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Caco-2, NT2/D1 and HT-29 whole cell lysate. Human pancreas lysate and fetal kidney lysate. IHC-P: Human pancreas tissue. Human pancreatic cancer tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20980-45 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226355于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 97 kDa.
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| IHC-P | (1) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 97 kDa. |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439). -
组织特异性
Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level). -
疾病相关
Retinitis pigmentosa 41
Cone-rod dystrophy 12
Stargardt disease 4
Retinal macular dystrophy 2 -
序列相似性
Belongs to the prominin family. -
翻译后修饰
Isoform 1 and isoform 2 are glycosylated.
Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis. -
细胞定位
Apical cell membrane. Cell projection, microvillus membrane. Cell projection, cilium, photoreceptor outer segment. Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine. - Information by UniProt
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数据库链接
- Entrez Gene: 8842 Human
- Omim: 604365 Human
- SwissProt: O43490 Human
- Unigene: 614734 Human
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别名
- AC133 antibody
- Antigen AC133 antibody
- CD133 antibody
see all
图片
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Western blot - Anti-CD133 antibody [EPR20980-45] (ab226355)All lanes : Anti-CD133 antibody [EPR20980-45] (ab226355) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate at 40 µg
Lane 2 : PROM1 knockout HAP1 cell lysate at 40 µg
Lane 3 : Caco-2 cell lysate at 20 µg
Lane 4 : HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesab226355 was shown to react with CD133 in wild-type HAP1 cells in western blot. Loss of signal was observed when PROM1 knockout sample was used. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab226355 overnight at 4°C at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1/5000 for 1 hour at room temperature before development with ECL reagent and imaging.
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![Western blot - Anti-CD133 antibody [EPR20980-45] (ab226355)](https://www.abcam.cn/ps/products/226/ab226355/Images/ab226355-287552-anti-cd133-antibody-epr20980-45-western-blot.jpg)
Western blot - Anti-CD133 antibody [EPR20980-45] (ab226355)All lanes : Anti-CD133 antibody [EPR20980-45] (ab226355) at 1/1000 dilution
Lane 1 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : NCCIT (human pluripotent embryonic carcinoma epithelial cell), whole cell lysate
Lane 4 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 97 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking: 5% NFDM/TBST.
The MW observed is consistent with the literature (PMID 20717901; PMID 18645205).
Negative control: HEK-293T (PMID 20167130).
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![Western blot - Anti-CD133 antibody [EPR20980-45] (ab226355)](https://www.abcam.cn/ps/products/226/ab226355/Images/ab226355-287551-anti-cd133-antibody-epr20980-45-western-blot.jpg)
Western blot - Anti-CD133 antibody [EPR20980-45] (ab226355)All lanes : Anti-CD133 antibody [EPR20980-45] (ab226355) at 1/1000 dilution
Lane 1 : Human pancreas lysate
Lane 2 : NT2/D1 (human malignant pluripotent embryonic carcinoma epithelial cell-like), whole cell lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 97 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Blocking: 5% NFDM/TBST.
Exposure times:
Lane 1: 3 minutes
Lanes 2-3: 10 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-45] (ab226355)](https://www.abcam.cn/ps/products/226/ab226355/Images/ab226355-287550-anti-cd133-antibody-epr20980-45-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-45] (ab226355)Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling CD133 with ab226355 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP), ready to use. Apical/endoluminal staining on ducts of human pancreas, and negative on Langerhans cells of the islets (PMID: 18261235) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP), ready to use.
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-45] (ab226355)](https://www.abcam.cn/ps/products/226/ab226355/Images/ab226355-287549-anti-cd133-antibody-epr20980-45-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-45] (ab226355)Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling CD133 with ab226355 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP), ready to use. Apical/endoluminal staining on ducts of human pancreatic cancer (PMID: 18261235) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP), ready to use.
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
![Anti-CD133 antibody [EPR20980-45] (ab226355)](https://www.abcam.cn/ps/products/226/ab226355/Images/ab226355-5-benefits-of-recombinant-antibodies.png)
Anti-CD133 antibody [EPR20980-45] (ab226355)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (9)
ab226355 被引用在 9 文献中.
- Han JY et al. Desmoglein 2 and desmocollin 2 depletions promote malignancy through distinct mechanisms in triple-negative and luminal breast cancer. BMC Cancer 24:532 (2024). WB ; Human . PubMed: 38671389
- Zhou T et al. Artificial intelligence-based comprehensive analysis of immune-stemness-tumor budding profile to predict survival of patients with pancreatic adenocarcinoma. Cancer Biol Med 20:196-217 (2023). PubMed: 36971107
- Tang B et al. Blood exosome marker miRNA-30d-5p: Role and regulation mechanism in cell stemness and gemcitabine resistance of hepatocellular carcinoma. Mol Cell Probes 71:101924 (2023). WB ; Human . PubMed: 37536457
- Wang Y et al. Overexpression of FOXD2-AS1 enhances proliferation and impairs differentiation of glioma stem cells by activating the NOTCH pathway via TAF-1. J Cell Mol Med 26:2620-2632 (2022). IHC-P ; Human . PubMed: 35419917
- Ai C et al. Nogo-A/NgR signaling regulates stemness in cancer stem-like cells derived from U87MG glioblastoma cells. Oncol Lett 24:230 (2022). WB ; Human . PubMed: 35720478
- Peng W et al. OCT4 and SOX2 Specific Cytotoxic T Cells Exhibit Not Only Good Efficiency but Also Synergize PD-1 Inhibitor (Nivolumab) in Treating Breast Cancer Stem-Like Cells and Drug-Resistant Breast Cancer Mice. Front Oncol 12:781093 (2022). PubMed: 35402219
- Gu X et al. ESM1/HIF‑1α pathway modulates chronic intermittent hypoxia‑induced non‑small‑cell lung cancer proliferation, stemness and epithelial‑mesenchymal transition. Oncol Rep 45:1226-1234 (2021). PubMed: 33650648
- Qiu GZ et al. Hypoxia-induced USP22-BMI1 axis promotes the stemness and malignancy of glioma stem cells via regulation of HIF-1α. Life Sci 247:117438 (2020). WB ; Human . PubMed: 32070708
- Ye T et al. TIPE1 impairs stemness maintenance in colorectal cancer through directly targeting ß-catenin. Carcinogenesis 41:25-35 (2020). WB ; Human . PubMed: 31111874